Somatic Embryogenesis of Brachiaria brizantha (Syn. Urochloa brizantha) Analyzed by In Situ Hybridization.
| Autor: | da Silveira SR; Plant Biotechnology Lab, Center of Nuclear Energy in Agriculture, University of São Paulo, Piracicaba, SP, Brazil., Koehler AD; Plant Biotechnology Lab, Center of Nuclear Energy in Agriculture, University of São Paulo, Piracicaba, SP, Brazil., Mendes Gomes ACM; Brazilian Agricultural Research Corporation (Embrapa), Embrapa Genetic Resources and Biotechnology, Brasilia, DF, Brazil., Cabral GB; Brazilian Agricultural Research Corporation (Embrapa), Embrapa Genetic Resources and Biotechnology, Brasilia, DF, Brazil., de Campos Carneiro VT; Brazilian Agricultural Research Corporation (Embrapa), Embrapa Genetic Resources and Biotechnology, Brasilia, DF, Brazil., de Alencar Dusi DM; Brazilian Agricultural Research Corporation (Embrapa), Embrapa Genetic Resources and Biotechnology, Brasilia, DF, Brazil. diva.dusi@embrapa.br., Martinelli AP; Plant Biotechnology Lab, Center of Nuclear Energy in Agriculture, University of São Paulo, Piracicaba, SP, Brazil. adriana@cena.usp.br. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2527, pp. 247-263. |
| DOI: | 10.1007/978-1-0716-2485-2_18 |
| Abstrakt: | In situ hybridization with mRNA probes enables the detection and localization of gene expression in plant somatic embryogenesis samples. BbrizSERK is a gene that is expressed in embryogenic cells and tissues of Brachiaria. Here we describe methods used for in situ hybridization to localize BbrizSERK transcripts during somatic embryogenesis of Brachiaria brizantha according to the plant material and observations intended, using paraffin or butyl methyl methacrylate resin-embedded samples, as well as a method for whole-mount preparation applicable for the analysis of other genes involved in embryogenic processes, along with other in vitro processes. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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