Electrospun polylactic acid scaffolds with strontium- and cobalt-doped bioglass for potential use in bone tissue engineering applications.

Autor: de Souza JR; Department of Dental Materials and Prosthodontics, Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), São José dos Campos, São Paulo, Brazil., Kukulka EC; Department of Dental Materials and Prosthodontics, Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), São José dos Campos, São Paulo, Brazil., Araújo JCR; Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), São José dos Campos, São Paulo, Brazil., Campos TMB; Technological Institute of Aeronautics (ITA), Praça Marechal Eduardo Gomes, São José dos Campos, São Paulo, Brazil., do Prado RF; Department of Social Dentistry and Children's Clinic, Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), São José dos Campos, São Paulo, Brazil., de Vasconcellos LMR; Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), São José dos Campos, São Paulo, Brazil., Thin GP; Technological Institute of Aeronautics (ITA), Praça Marechal Eduardo Gomes, São José dos Campos, São Paulo, Brazil., Borges ALS; Department of Dental Materials and Prosthodontics, Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), São José dos Campos, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part B, Applied biomaterials [J Biomed Mater Res B Appl Biomater] 2023 Jan; Vol. 111 (1), pp. 151-160. Date of Electronic Publication: 2022 Aug 11.
DOI: 10.1002/jbm.b.35141
Abstrakt: The development of nanoscale biomaterials associated with polymers has been growing over the years, due to their important structural characteristics for applications in biological systems. The present study aimed to produce and test polymeric scaffolds composed of polylactic acid (PLA) fibers associated with a 58S bioglass doped with therapeutic ions for use in tissue engineering. Three 58S Bioglass was obtained by the sol-gel route, pure and doped with 5% strontium and cobalt ions. Solutions of 7% PLA was used as control and added the three different bioglass, 4% of 58S bioglass (PLA-BG), 4% bioglass-doped strontium (PLA-BGSr) and 4% bioglass-doped cobalt (PLA-BGCo). Scaffolds were produced through electrospinning process, and was characterized chemical and morphologically. The in vitro tests were performed using mesenchymal cells cultures from femurs of nine rats, grown in osteogenic supplemented total culture medium. After osteoblastic differentiation induction cell viability, alkaline phosphatase activity, total protein content quantification, and visualization of mineralization nodule tests were performed. Analysis of normal distribution used the Shapiro-Wilk test (nanofibers diameter and biological assay). Data were compared using the Kruskal-Wallis nonparametric test (p = 0.05). The bioglasses produced proved to be free of nitrate, chlorinated and nano-sized, with effective incorporation of therapeutic ions in their structure. All materials showed cell viability (>70%), total protein production, and alkaline phosphatase activity. It was possible to develop polylactic acid scaffolds associated with 58S bioglass doped with therapeutic ions without cytotoxicity. Scaffolds characteristics appear to sustain its application in bone tissue engineering.
(© 2022 Wiley Periodicals LLC.)
Databáze: MEDLINE
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