Anti-inflammatory withajardins from the leaves of Athenaea velutina.

Autor: Dantas Rocha KA; Departamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, 60021-970, Fortaleza, CE, Brazil., de Freitas Paulo T; Departamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, 60021-970, Fortaleza, CE, Brazil., Ayala AP; Departamento de Física, Centro de Ciências, Universidade Federal do Ceará, 60440-900, Fortaleza, CE, Brazil., da Silva Sampaio V; Ciências Biológicas, Universidade Regional do Cariri, 63200-000, Missão Velha, CE, Brazil., Gomes Nunes PI; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, 60430-270, Fortaleza, CE, Brazil., Santos FA; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, 60430-270, Fortaleza, CE, Brazil., Canuto KM; Embrapa Agroindústria Tropical, 60511-110, Fortaleza, CE, Brazil., Silveira ER; Departamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, 60021-970, Fortaleza, CE, Brazil., Loiola Pessoa OD; Departamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, 60021-970, Fortaleza, CE, Brazil. Electronic address: opessoa@ufc.br.
Jazyk: angličtina
Zdroj: Phytochemistry [Phytochemistry] 2022 Nov; Vol. 203, pp. 113338. Date of Electronic Publication: 2022 Aug 07.
DOI: 10.1016/j.phytochem.2022.113338
Abstrakt: Withajardins, uncommon modified withanolide-type steroids, have been isolated exclusively from plants of the Solanaceae family so far. Two undescribed withajardins and the known tuboanosigenin were isolated from the hexane/EtOAc 1:1 extract from Athenaea velutina leaves. Their structures were established by an extensive analysis of 1D and 2D-NMR and HRMS data. The absolute configuration was determined by X-ray diffraction (withajardin L and tuboanosigenin) and circular dichroism (CD) analyses (withajardin M). The anti-inflammatory activity of compounds was evaluated through the inhibition of the lipopolysaccharide (LPS)-induced nitric oxide (NO), TNF-α, and IL-6 release in RAW264.7 cells. The cell viability effects to RAW 264.7 cells showed IC 50 values of 74.4-354.4 μM. The compounds attenuated LPS-induced release of NO and decreased pro-inflammatory cytokines TNF-α and IL-6 in RAW264.7 cells.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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