Expression pattern of microRNAs in ovine endometrium during the peri-implantation.

Autor: Kose M; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Dicle University, Diyarbakir, Turkey., Hitit M; Department of Genetics, Faculty of Veterinary Medicine, Kastamonu University, Kastamonu, Turkey., Kaya MS; Department of Physiology, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara, Turkey., Kırbas M; Bahri Dagdas International Agricultural Research Institute, Konya, Turkey., Dursun S; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Aksaray University, Aksaray, Turkey., Alak I; Department of Animal Sciences, Vocational School of Technical Sciences, Ankara Yıldırım Beyazıt University, Ankara, Turkey., Atli MO; Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Harran University, Sanliurfa, Turkey. Electronic address: moatli@hotmail.com.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2022 Oct 01; Vol. 191, pp. 35-46. Date of Electronic Publication: 2022 Jul 31.
DOI: 10.1016/j.theriogenology.2022.07.015
Abstrakt: MicroRNA (miRNA), acting as the transcriptional regulator of gene expression, has been widely demonstrated to be involved in many biological functions, including embryo implantation and development. The objective of the current study was to illuminate the expression pattern of microRNAs (miRNAs) in the endometrium during the peri-implantation in ewes. Intercaruncular endometrial samples was obtained from a total of 24 ewes on days of 12 (pre-implantation, n = 4), 16 (implantation, n = 4) and 22 (post-implantation, n = 4) of pregnancy following mating, and on their corresponding days of 12 (n = 4), 16 (n = 4) and 22 (n = 4) of the estrous cycle. The miRNA profiles were examined in the endometrium by microarray technology. We detected 116 ovine specifics miRNAs in the endometrium. Of these, nineteen were differentially expressed in early pregnancy. Four miRNAs (oar-miR-370-3p, oar-miR-411b-5p, oar-miR-379-3p and oar-miR-411a-3p) that had the most differential fold change were confirmed by RT-qPCR in ovine endometrium. The differentially expressed miRNAs targeted a total of 315 genes, resulting in 39 GO terms in molecular function, 353 in biological process, and 17 in the cellular component. The construction of the PPI network of target genes established two functional modules mostly enriched in the innate immune system, toll receptor cascades in module 1, whereas genes in module 2 were associated with GMCSF-mediated signaling events, insulin pathway, and mTOR signaling pathway. Based on the results, we may imply that miRNAs modulate ovine endometrium during the peri-implantation.
Competing Interests: Declaration of competing interest The authors declare no competing interests.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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