Circulation Time-Optimized Albumin Nanoplatform for Quantitative Visualization of Lung Metastasis via Targeting of Macrophages.

Autor:	Chung H; Macrophage Lab, Department of Microbiology and Immunology, and Institute of Endemic Disease, Seoul National University College of Medicine, Seoul 03080, Republic of Korea.; Bio-MAX Institute, Seoul National University, Seoul 03080, Republic of Korea., Park JY; Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Republic of Korea.; Department of Nuclear Medicine, Seoul National University Hospital, Seoul 03080, Republic of Korea.; Cancer Research Institute, Seoul National University, Seoul 03080, Republic of Korea.; Dental Research Institute, Seoul National University, Seoul 03080, Republic of Korea., Kim K; Department of Nuclear Medicine, Seoul National University Hospital, Seoul 03080, Republic of Korea.; Cancer Research Institute, Seoul National University, Seoul 03080, Republic of Korea., Yoo RJ; Department of Nuclear Medicine, Seoul National University Hospital, Seoul 03080, Republic of Korea.; Cancer Research Institute, Seoul National University, Seoul 03080, Republic of Korea., Suh M; Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 03080, Republic of Korea., Gu GJ; Macrophage Lab, Department of Microbiology and Immunology, and Institute of Endemic Disease, Seoul National University College of Medicine, Seoul 03080, Republic of Korea., Kim JS; Department of Nuclear Medicine, Seoul National University Hospital, Seoul 03080, Republic of Korea., Choi TH; Department of Nuclear Medicine, Seoul National University Hospital, Seoul 03080, Republic of Korea.; Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 03080, Republic of Korea., Byun JW; Department of Nuclear Medicine, Seoul National University Hospital, Seoul 03080, Republic of Korea., Ju YW; Department of Surgery and Cancer Research Institute, Seoul National University College of Medicine, Seoul 03080, Republic of Korea., Han W; Department of Surgery and Cancer Research Institute, Seoul National University College of Medicine, Seoul 03080, Republic of Korea., Ryu HS; Department of Pathology, Seoul National University College of Medicine, Seoul 03080, Republic of Korea., Chung G; Dental Research Institute, Seoul National University, Seoul 03080, Republic of Korea.; Department of Oral Physiology, Seoul National University, School of Dentistry, Seoul 03080, Republic of Korea., Hwang DW; Department of Nuclear Medicine, Seoul National University Hospital, Seoul 03080, Republic of Korea.; Research and Development Center, THERABEST, Co. Ltd., Seoul 03080, Republic of Korea., Kim Y; Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Republic of Korea., Kang HR; Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Republic of Korea., Na YR; Transdisciplinary Department of Medicine and Advanced Technology, Seoul National University Hospital, Seoul 03080, Republic of Korea., Choi H; Department of Nuclear Medicine, Seoul National University Hospital, Seoul 03080, Republic of Korea., Im HJ; Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 03080, Republic of Korea.; Research Institute for Convergence Science, Seoul National University, Seoul 08823, Republic of Korea., Lee YS; Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Republic of Korea.; Department of Nuclear Medicine, Seoul National University Hospital, Seoul 03080, Republic of Korea.; Cancer Research Institute, Seoul National University, Seoul 03080, Republic of Korea., Seok SH; Macrophage Lab, Department of Microbiology and Immunology, and Institute of Endemic Disease, Seoul National University College of Medicine, Seoul 03080, Republic of Korea.; Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Republic of Korea.
Jazyk:	angličtina
Zdroj:	ACS nano [ACS Nano] 2022 Aug 23; Vol. 16 (8), pp. 12262-12275. Date of Electronic Publication: 2022 Aug 09.
DOI:	10.1021/acsnano.2c03075
Abstrakt:	The development of molecular imaging probes to identify key cellular changes within lung metastases may lead to noninvasive detection of metastatic lesions in the lung. In this study, we constructed a macrophage-targeted clickable albumin nanoplatform (CAN) decorated with mannose as the targeting ligand using a click reaction to maintain the intrinsic properties of albumin in vivo . We also modified the number of mannose molecules on the CAN and found that mannosylated serum albumin (MSA) harboring six molecules of mannose displayed favorable pharmacokinetics that allowed high-contrast imaging of the lung, rendering it suitable for in vivo visualization of lung metastases. Due to the optimized control of functionalization and surface modification, MSA enhanced blood circulation time and active/passive targeting abilities and was specifically incorporated by mannose receptor (CD206)-expressing macrophages in the metastatic lung. Moreover, extensive in vivo imaging studies using single-photon emission computed tomography (SPECT)/CT and positron emission tomography (PET) revealed that blood circulation of time-optimized MSA can be used to discern metastatic lesions, with a strong correlation between its signal and metastatic burden in the lung.
Databáze:	MEDLINE
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