Luteolin-Rich Extract of Thespesia garckeana F. Hoffm. (Snot Apple) Contains Potential Drug-Like Candidates and Modulates Glycemic and Oxidoinflammatory Aberrations in Experimental Animals.

Autor: Alozieuwa UB; Department of Biochemistry, Faculty of Natural and Applied Sciences, Veritas University Abuja, FCT-Abuja, Nigeria., Lawal B; PhD Program for Cancer Molecular Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei and Academia Sinica, Taipei 11031, Taiwan.; Graduate Institute for Cancer Biology & Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan., Sani S; Department of Biochemistry and Molecular Biology, Faculty of Science, Federal University Ndufu-Alike Ikwo, P.M., B 1010 Abakaliki, Ebonyi State., Nigeria., Onikanni AS; Department of Chemical Sciences, Biochemistry Unit, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria.; College of Medicine, Graduate Institute of Biomedical Science, China Medical University, Taiwan., Osuji O; Department of Chemistry, Faculty of Physical Sciences, Alex-Ekwueme Federal University Ndufu-Alike, Ebonyi State, Nigeria., Ibrahim YO; Department of Biochemistry, Federal University of Technology, Minna, Nigeria., Babalola SB; Department of Chemistry, Federal University of Technology, Minna, Nigeria., Mostafa-Hedeab G; Pharmacology Department & Health Research Unit, Medical College Jouf University, Jouf, Saudi Arabia.; Pharmacology Department Faculty of Medicine, Beni-Suef University, Egypt., Alsayegh AA; Clinical Nutrition Department, Applied Medical Sciences College, Jazan University, Jazan 82817, Saudi Arabia., Albogami S; Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia., Batiha GE; Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt., Wu ATH; TMU Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 11031, Taiwan.; The PhD Program of Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan.; Clinical Research Center, Taipei Medical University Hospital, Taipei Medical University, Taipei 11031, Taiwan.; Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei 11490, Taiwan., Huang HS; PhD Program for Cancer Molecular Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei and Academia Sinica, Taipei 11031, Taiwan.; Graduate Institute for Cancer Biology & Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan.; School of Pharmacy, National Defense Medical Center, Taipei 11490, Taiwan.; PhD Program in Biotechnology Research and Development College of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan., Conte-Junior CA; Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ), Cidade Universitária, Rio de Janeiro 21941-598, Brazil.
Jazyk: angličtina
Zdroj: Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2022 Jul 30; Vol. 2022, pp. 1215097. Date of Electronic Publication: 2022 Jul 30 (Print Publication: 2022).
DOI: 10.1155/2022/1215097
Abstrakt: The present study evaluated the polyphenolic contents and hypoglycemic, antioxidant, and anti-inflammatory effects of the diethyl ether fraction of Thespesia garckeana using various in vitro and in vivo models. Total phenol and flavonoid contents of the extract were 613.65 ± 2.38 and 152.83 ± 1.56 mg/100 g dry weight, respectively. The extract exhibited in vitro antioxidant activities against DPPH, FRAP, LPO, and ABTS with respective half-maximal inhibitory concentration (IC 50 ) values of 30.91 ± 0.23, 16.81 ± 0.51, 41.29 ± 1.82, and 42.39 ± 2.24  μ g/mL. In vitro anti-inflammatory studies using membrane stabilization, protein denaturation, and proteinase activities revealed the effectiveness of the extract with respective IC 50 values of 54.45 ± 2.89, 93.62 ± 3.04, and 56.60 ± 2.34  μ g/mL, while in vitro hypoglycemic analysis of the extract revealed inhibition of α -amylase (IC 50 64.59 ± 3.29  μ g/mL) and enhancement of glucose uptake by yeast cells. Interestingly, the extract demonstrated in vivo hypoglycemic and anti-inflammatory effects in streptozotocin- (STZ-) induced diabetic and xylene-induced ear swelling models, respectively. In addition, the extract improved insulin secretion, attenuated pancreatic tissue distortion and oxidative stress, and increased the activities of superoxide dismutase (SOD), catalase, and reduced glutathione (GSH), while reducing the concentration of LPO in the diabetic rats. A high-performance liquid chromatography (HPLC) analysis identified the presence of catechin (6.81 e - 1 ppm), rutin (8.46  e - 1 ppm), myricetin, apigenin (4.019  e - 1 ppm), and luteolin (15.09 ppm) with respective retention times (RTs) of 13.64, 24.269, 27.781, 29.58, and 32.23 min, and these were subjected to a pharmacoinformatics analysis, which revealed their drug-likeness and good pharmacokinetic properties. A docking analysis hinted at the potential of luteolin, the most abundant compound in the extract, for targeting glucose-metabolizing enzymes. Thus, the present study provides preclinical insights into the bioactive constituents of T. garckeana , its antioxidant and anti-inflammatory effects, and its potential for the treatment of diabetes.
Competing Interests: The authors declare no conflict of interest.
(Copyright © 2022 Uchenna Blessing Alozieuwa et al.)
Databáze: MEDLINE
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