First report and whole-genome sequencing of Pseudochrobactrum saccharolyticum in Latin America.

Autor: Pereira CR; Departamento de Medicina Veterinária, Faculdade de Zootecnia e Medicina Veterinária, Universidade Federal de Lavras, Lavras, Minas Gerais, Brazil., de Jesus Sousa T; Departamento de Genética, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Lima da Silva A; Departamento de Genética, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Gonçalves Dos Santos R; Departamento de Genética, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Minharro S; Centro de Ciência da Saúde - Medicina - Araguaína, Universidade Federal de Tocantins, Tocantins, Brazil., Costa Custódio DA; Departamento de Medicina Veterinária, Faculdade de Zootecnia e Medicina Veterinária, Universidade Federal de Lavras, Lavras, Minas Gerais, Brazil., Pickard DJ; Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom., O'Callaghan D; IVBIC, INSERM, Universite de Montpellier, Nimes, France; CNR Brucella, Laboratoire de Microbiologie, CHU Nimes, Nimes, France., Foster JT; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., de Castro Soares S; Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Biológicas e Ciências Naturais, Universidade Federal Do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil., Juca Ramos RT; Instituto de Ciências Biológicas, Centro de Genômica e Biologia de Sistemas, Universidade Federal Do Pará, Belém, Pará, Brazil., Góes-Neto A; Departamento de Genética, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Matiuzzi da Costa M; Universidade Federal Do Vale Do São Francisco, Departamento de Zootecnia, Petrolina, Pernambuco, Brazil., Lage AP; Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Azevedo V; Departamento de Genética, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Seles Dorneles EM; Departamento de Medicina Veterinária, Faculdade de Zootecnia e Medicina Veterinária, Universidade Federal de Lavras, Lavras, Minas Gerais, Brazil. Electronic address: elaine.dorneles@ufla.br.
Jazyk: angličtina
Zdroj: Microbes and infection [Microbes Infect] 2023 Jan-Feb; Vol. 25 (1-2), pp. 105018. Date of Electronic Publication: 2022 Aug 05.
DOI: 10.1016/j.micinf.2022.105018
Abstrakt: The Brucellaceae family comprises microorganisms similar both phenotypically and genotypically, making it difficult to identify the etiological agent of these infections. This study reports the first isolation, identification, and characterization of Pseudochrobactrum saccharolyticum (strain 115) from Latin America. Strain 115 was isolated in 2007 from a bovine in Brazil and was initially classified as Brucella spp. by classical microbiological tests and bcsp31 PCR. The antimicrobial susceptibility of strain 115 was tested against drugs used to treat human brucellosis by minimal inhibitory concentration test. Subsequently, the whole genome of the strain was sequenced, assembled, and characterized. Phylogenetic trees built from 16S rRNA and recA gene sequences enabled the classification of strain 115 as Pseudochrobactrum spp. Phylogenomic analysis using Single Nucleotide Polymorphisms and Average Nucleotide Identity allowed the classification of the strain as P. saccharolyticum. Additionally, a Tetra Correlation Search identified one related genome from the same species, which was compared with strain 115 by analyzing genomic islands. This is the first identification and whole-genome sequence of P. saccharolyticum in Latin America and highlights a challenge in the diagnosis of bovine brucellosis, which could be solved by including the sequencing of 16S rRNA and recA genes in routine diagnostics.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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