| Autor: |
Maekawa T; Department of Rehabilitation for Motor Functions, National Rehabilitation Center for Persons with Disabilities., Sakitani N; Department of Rehabilitation for Motor Functions, National Rehabilitation Center for Persons with Disabilities., Ryu Y; Department of Rehabilitation for Motor Functions, National Rehabilitation Center for Persons with Disabilities., Takashima A; Department of Assistive Technology, National Rehabilitation Center for Persons with Disabilities., Murase S; Department of Rehabilitation for Motor Functions, National Rehabilitation Center for Persons with Disabilities., Fink J; Department of Metabolism and Endocrinology, Graduate School of Medicine, Juntendo University., Nagao M; Department of Rehabilitation for Motor Functions, National Rehabilitation Center for Persons with Disabilities., Ogata T; Department of Rehabilitation Medicine, Graduate School of Medicine, The University of Tokyo., Shinohara M; Department of Rehabilitation for Motor Functions, National Rehabilitation Center for Persons with Disabilities., Sawada Y; Department of Clinical Research, National Rehabilitation Center for Persons with Disabilities; ys454-ind@umin.ac.jp. |
| Abstrakt: |
Exercise is widely recognized as effective for various diseases and physical disorders, including those related to brain dysfunction. However, molecular mechanisms behind the beneficial effects of exercise are poorly understood. Many physical workouts, particularly those classified as aerobic exercises such as jogging and walking, produce impulsive forces at the time of foot contact with the ground. Therefore, it was speculated that mechanical impact might be implicated in how exercise contributes to organismal homeostasis. For testing this hypothesis on the brain, a custom-designed ''passive head motion'' (hereafter referred to as PHM) system was developed that can generate vertical accelerations with controlled and defined magnitudes and modes and reproduce mechanical stimulation that might be applied to the heads of rodents during treadmill running at moderate velocities, a typical intervention to test the effects of exercise in animals. By using this system, it was demonstrated that PHM recapitulates the serotonin (5-hydroxytryptamine, hereafter referred to as 5-HT) receptor subtype 2A (5-HT2A) signaling in the prefrontal cortex (PFC) neurons of mice. This work provides detailed protocols for applying PHM and measuring its resultant mechanical accelerations at rodents' heads. |
