Exploring the role of antimicrobials in the selective growth of purple phototrophic bacteria through genome mining and agar spot assays.

Autor: Alloul A; Department of Biotechnology, Delft University of Technology, Delft, the Netherlands.; Department of Bioscience Engineering, University of Antwerp, Antwerpen, Belgium., Van Kampen W; Department of Biotechnology, Delft University of Technology, Delft, the Netherlands., Cerruti M; Department of Biotechnology, Delft University of Technology, Delft, the Netherlands., Wittouck S; Department of Bioscience Engineering, University of Antwerp, Antwerpen, Belgium., Pabst M; Department of Biotechnology, Delft University of Technology, Delft, the Netherlands., Weissbrodt DG; Department of Biotechnology, Delft University of Technology, Delft, the Netherlands.
Jazyk: angličtina
Zdroj: Letters in applied microbiology [Lett Appl Microbiol] 2022 Nov; Vol. 75 (5), pp. 1275-1285. Date of Electronic Publication: 2022 Aug 08.
DOI: 10.1111/lam.13795
Abstrakt: Purple non-sulphur bacteria (PNSB) are an emerging group of microbes attractive for applied microbiology applications such as wastewater treatment, plant biostimulants, microbial protein, polyhydroxyalkanoates and H 2 production. These photoorganoheterotrophic microbes have the unique ability to grow selectively on organic carbon in anaerobic photobioreactors. This so-called selectivity implies that the microbial community will have a low diversity and a high abundance of a particular PNSB species. Recently, it has been shown that certain PNSB strains can produce antimicrobials, yet it remains unclear whether these contribute to competitive inhibition. This research aimed to understand which type of antimicrobial PNSB produce and identify whether these compounds contribute to their selective growth. Mining 166 publicly-available PNSB genomes using the computational tool BAGEL showed that 59% contained antimicrobial encoding regions, more specifically biosynthetic clusters of bacteriocins and non-ribosomal peptide synthetases. Inter- and intra-species inhibition was observed in agar spot assays for Rhodobacter blasticus EBR2 and Rhodopseudomonas palustris EBE1 with inhibition zones of, respectively, 5.1 and 1.5-5.7 mm. Peptidomic analysis detected a peptide fragment in the supernatant (SVLQLLR) that had a 100% percentage identity match with a known non-ribosomal peptide synthetase with antimicrobial activity.
(© 2022 The Authors. Letters in Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.)
Databáze: MEDLINE
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