A UV-C LED-based unit for continuous decontamination of the sheath fluid in a flow-cytometric cell sorter.

Autor: Kirsch J; Flow Cytometry Core Facility Deutsches Rheuma-Forschungszentrumein Institut der Leibniz-Gemeinschaft Berlin Germany., Heinrich K; Flow Cytometry Core Facility Deutsches Rheuma-Forschungszentrumein Institut der Leibniz-Gemeinschaft Berlin Germany., Kage D; Flow Cytometry Core Facility Deutsches Rheuma-Forschungszentrumein Institut der Leibniz-Gemeinschaft Berlin Germany., Glaab J; Ferdinand-Braun-Institut gGmbH Leibniz-Institut für Höchstfrequenztechnik Berlin Germany., Grothe B; APE Angewandte Physik und Elektronik GmbH Berlin Germany., Volkmann KV; APE Angewandte Physik und Elektronik GmbH Berlin Germany., Kaiser T; Flow Cytometry Core Facility Deutsches Rheuma-Forschungszentrumein Institut der Leibniz-Gemeinschaft Berlin Germany.
Jazyk: angličtina
Zdroj: Engineering in life sciences [Eng Life Sci] 2022 Jul 08; Vol. 22 (8), pp. 550-553. Date of Electronic Publication: 2022 Jul 08 (Print Publication: 2022).
DOI: 10.1002/elsc.202200025
Abstrakt: Aseptic cell sorting is challenging, especially when a flow-cytometric cell sorter is not operated in a sterile environment. The sheath fluid system of a cell sorter may be contaminated with germs such as bacteria, yeasts, viruses, or fungi. Thus, a regular chemical cleaning procedure is required to prepare a sorter for aseptic cell sorting by flushing the fluidic system. However, this procedure is time consuming, and most importantly, the researcher can never be sure that the cleaning process was successful. Here we present a method in which the sheath fluid of a cell sorter was decontaminated by irradiation with UV-C light using a flow-through principle. Using this principle, we were able to achieve a 5 log reduction of bacteria in the sheath fluid.
Competing Interests: K.v.V and B.G. are employees of APE. All other authors declare no commercial or financial conflict of interest.
(© 2022 The Authors. Engineering in Life Sciences published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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