Human Pluripotent Stem Cell-Derived Micropatterned Ectoderm Allows Cell Sorting of Meso-Endoderm Lineages.

Autor:	Yang Y; Department of Industrial Engineering (DII), University of Padova, Padova, Italy.; Fondazione Ricerca Biomedica Avanzata Onlus, Veneto Institute of Molecular Medicine, Padova, Italy., Laterza C; Department of Industrial Engineering (DII), University of Padova, Padova, Italy.; Fondazione Ricerca Biomedica Avanzata Onlus, Veneto Institute of Molecular Medicine, Padova, Italy., Stuart HT; Department of Industrial Engineering (DII), University of Padova, Padova, Italy.; Fondazione Ricerca Biomedica Avanzata Onlus, Veneto Institute of Molecular Medicine, Padova, Italy., Michielin F; Fondazione Ricerca Biomedica Avanzata Onlus, Veneto Institute of Molecular Medicine, Padova, Italy.; Great Ormond Street Institute of Child Health, University College London, London, UK., Gagliano O; Department of Industrial Engineering (DII), University of Padova, Padova, Italy.; Fondazione Ricerca Biomedica Avanzata Onlus, Veneto Institute of Molecular Medicine, Padova, Italy., Urciuolo A; Fondazione Ricerca Biomedica Avanzata Onlus, Veneto Institute of Molecular Medicine, Padova, Italy.; Department of Molecular Medicine, University of Padova, Padova, Italy.; Istituto di Ricerca Pediatrica, Città della Speranza, Padova, Italy., Elvassore N; Department of Industrial Engineering (DII), University of Padova, Padova, Italy.; Fondazione Ricerca Biomedica Avanzata Onlus, Veneto Institute of Molecular Medicine, Padova, Italy.; Great Ormond Street Institute of Child Health, University College London, London, UK.
Jazyk:	angličtina
Zdroj:	Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2022 Jul 22; Vol. 10, pp. 907159. Date of Electronic Publication: 2022 Jul 22 (Print Publication: 2022).
DOI:	10.3389/fbioe.2022.907159
Abstrakt:	The human developmental processes during the early post-implantation stage instruct the specification and organization of the lineage progenitors into a body plan. These processes, which include patterning, cell sorting, and establishment of the three germ layers, have been classically studied in non-human model organisms and only recently, through micropatterning technology, in a human-specific context. Micropatterning technology has unveiled mechanisms during patterning and germ layer specification; however, cell sorting and their segregation in specific germ layer combinations have not been investigated yet in a human-specific in vitro system. Here, we developed an in vitro model of human ectodermal patterning, in which human pluripotent stem cells (hPSCs) self-organize to form a radially regionalized neural and non-central nervous system (CNS) ectoderm. We showed that by using micropatterning technology and by modulating BMP and WNT signals, we can regulate the appearance and spatial distribution of the different ectodermal populations. This pre-patterned ectoderm can be used to investigate the cell sorting behavior of hPSC-derived meso-endoderm cells, with an endoderm that segregates from the neural ectoderm. Thus, the combination of micro-technology with germ layer cross-mixing enables the study of cell sorting of different germ layers in a human context. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Yang, Laterza, Stuart, Michielin, Gagliano, Urciuolo and Elvassore.)
Databáze:	MEDLINE
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