Oxidative Stress Inhibition Via Apocynin Prevents Medullary Respiratory Neurodegeneration and Respiratory Pattern Dysfunction in a 6-Hydroxydopamine Animal Model of Parkinson's Disease.

Autor: Nascimento ALF; Departamento de Farmacologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, SP 05508-000, Brazil., Medeiros POS; Departamento de Farmacologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, SP 05508-000, Brazil., Pedrão LFAT; Departamento de Farmacologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, SP 05508-000, Brazil., Queiroz VC; Departamento de Ciencias Fisiológicas, Faculdade de Ciencias Medicas da Santa Casa de São Paulo, 01221-021, Brazil., Oliveira LM; Departamento de Farmacologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, SP 05508-000, Brazil., Novaes LS; Departamento de Farmacologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, SP 05508-000, Brazil., Caetano AL; Departamento de Ciencias Fisiológicas, Faculdade de Ciencias Medicas da Santa Casa de São Paulo, 01221-021, Brazil., Munhoz CD; Departamento de Farmacologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, SP 05508-000, Brazil., Takakura AC; Departamento de Farmacologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, SP 05508-000, Brazil., Falquetto B; Departamento de Farmacologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, SP 05508-000, Brazil. Electronic address: barbaraf@icb.usp.br.
Jazyk: angličtina
Zdroj: Neuroscience [Neuroscience] 2022 Oct 15; Vol. 502, pp. 91-106. Date of Electronic Publication: 2022 Aug 04.
DOI: 10.1016/j.neuroscience.2022.07.034
Abstrakt: Parkinson's Disease (PD) is a neurogenerative disorder characterized by the death of dopaminergic neurons in the Substantia Nigra pars compacta (SNpc), leading to motor, cognitive, learning, and respiratory dysfunctions. New evidence revealed that breathing impairment in PD mainly results from oxidative stress (OS) that initiates apoptotic signaling in respiratory neurons. Here, we investigated the role of OS inhibition using apocynin (non-specific NADPH oxidase inhibitor) in a 6-OHDA PD animal model in the neural control of breathing. The PD model was confirmed with a 70% reduction in TH-expressing neurons within the SNpc. After 20 and 40 days of PD induction, no differences were observed in superoxide anion levels in any respiratory nuclei. At 30 days after PD induction, 6-OHDA animals presented OS that was prevented in all respiratory nuclei by adding apocynin to the drinking water for 10 days. Forty days after PD animal model induction, impaired motor and breathing function, reduced Phox2b and NK1 receptors-expressing neurons in the medullary respiratory areas; decreased latency to fall in the rotarod motor test; and attenuated respiratory frequency and minute ventilation parameters at rest and under hypercapnia conditions were observed. After 20 days of apocynin treatment, neurodegeneration of respiratory nuclei and breathing dysfunction in 6-OHDA animals were prevented. Thus, OS contributes to respiratory neuron death, consequently leading to breathing dysfunction in the 6-OHDA PD animal model. Furthermore, these results present a new perspective for preventing the onset and progression of PD-related respiratory impairments.
(Copyright © 2022 IBRO. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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