Nanoplastics alter the conformation and activity of human serum albumin.

Autor: Rajendran D; Centre for Nanobiotechnology, Vellore Institute of Technology, Vellore 632014, Tamil Nadu, India., Chandrasekaran N; Centre for Nanobiotechnology, Vellore Institute of Technology, Vellore 632014, Tamil Nadu, India. Electronic address: nchandrasekaran@vit.ac.in., Waychal Y; Centre for Nanobiotechnology, Vellore Institute of Technology, Vellore 632014, Tamil Nadu, India., Mukherjee A; Centre for Nanobiotechnology, Vellore Institute of Technology, Vellore 632014, Tamil Nadu, India.
Jazyk: angličtina
Zdroj: NanoImpact [NanoImpact] 2022 Jul; Vol. 27, pp. 100412. Date of Electronic Publication: 2022 Aug 05.
DOI: 10.1016/j.impact.2022.100412
Abstrakt: Nanoplastics finds its presence in most of the consumer products. Their chance of coming in contact with human cells and components is rampant. This study focuses on the interaction of polystyrene nanoplastics (PSNPs) with human serum albumin (HSA), ultimately causing structural and functional properties of the protein. Fluorescence and UV-Visible spectroscopic studies reported that PSNPs form a spontaneous ground-state complex with HSA, by hydrogen bonding, van der waal's, and hydrophobic force of attraction. This causes changes in the environment around major aromatic amino acids, especially tryptophan-214, which has a strong affinity with PSNPs. Further docking analysis confirmed hydrophobic interactions between PSNPs and aromatic amino acids in subdomain IIA of HSA. A shift in amide bands in HSA, as determined by FTIR analysis confirmed the disturbances in its secondary structure followed by reordering which will lead to the unfolding of HSA. Besides, PSNPs reduce the esterase activity of HSA by competitive inhibition. This molecular-level information such as binding energy, binding site, binding forces, reversible or irreversible binding, and structural changes of protein will shed light on the extent of toxicity in humans. This study will emphasize the urgent need for regulation of the use of nanoplastics (NPs) in consumer products, as well as the need for more research to determine the fate of NPs in the biological system.
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Databáze: MEDLINE
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