Transcript analysis and expression of the glbO gene, encoding truncated hemoglobin,O, of M. Smegmatis implicate its role under hypoxia and oxidative stress.

Autor: Chhaya A; Department of Biotechnology, Panjab University, Chandigarh 160014, India., Sharma A; Department of Biotechnology, Panjab University, Chandigarh 160014, India., Dattu Hade M; Department of Biotechnology, Panjab University, Chandigarh 160014, India., Kaur J; Department of Biotechnology, Panjab University, Chandigarh 160014, India., Dikshit KL; Department of Biotechnology, Panjab University, Chandigarh 160014, India. Electronic address: kanakdikshit@pu.ac.in.
Jazyk: angličtina
Zdroj: Gene [Gene] 2022 Oct 20; Vol. 841, pp. 146759. Date of Electronic Publication: 2022 Aug 03.
DOI: 10.1016/j.gene.2022.146759
Abstrakt: Although truncated hemoglobin O, (trHbO), is ubiquitous among mycobacteria, its physiological function is not very obvious and may be diverse. In an attempt to understand role of trHbO in cellular metabolism of a non-pathogenic mycobacterium, we analysed expression profile of the glbO gene, encoding trHbO, in M. smegmatis and studied implications of its overexpression on physiology of its host under different environmental conditions. Quantitative RT-PCR indicated that transcript level of the glbO gene remains low at a basal level under aerobic growth cycle of M. smegmatis but its level gets induced significantly during low oxygen, oxidative stress and macrophage infection. Overexpression of the glbO gene enhanced growth of M. smegmatis under hypoxia, promoted pellicle biofilm formation and provided resistance towards oxidative stress. Additionally, glbO gene overexpressing M. smegmatis exhibited enhanced cell survival over isogenic control cells and altered the level of pro- and anti- inflammatory cytokines during intracellular infection. These results suggested important role of trHbO, in supporting the cellular metabolism and survival of M, smegmatis both under low oxygen and oxidative stress.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: