Skeletal muscle macrophage ablation in mice.

Autor: Krall JTW; Department of Internal Medicine, Section on Pulmonary, Critical Care, Allergy, and Immunologic Diseases, Wake Forest School of Medicine, Winston-Salem, NC, United States. Electronic address: jkrall@wakehealth.edu., Gibbs KW; Department of Internal Medicine, Section on Pulmonary, Critical Care, Allergy, and Immunologic Diseases, Wake Forest School of Medicine, Winston-Salem, NC, United States., Belfield L; Department of Internal Medicine, Section on Pulmonary, Critical Care, Allergy, and Immunologic Diseases, Wake Forest School of Medicine, Winston-Salem, NC, United States., Liu C; Department of Internal Medicine, Section on Pulmonary, Critical Care, Allergy, and Immunologic Diseases, Wake Forest School of Medicine, Winston-Salem, NC, United States., Purcell L; Department of Internal Medicine, Section on Pulmonary, Critical Care, Allergy, and Immunologic Diseases, Wake Forest School of Medicine, Winston-Salem, NC, United States., Bivona JJ; Department of Medicine, University of Vermont Larner College of Medicine, Burlington, VT, United States., Poynter ME; Department of Medicine, University of Vermont Larner College of Medicine, Burlington, VT, United States., Stapleton RD; Department of Medicine, University of Vermont Larner College of Medicine, Burlington, VT, United States., Toth MJ; Department of Medicine, University of Vermont Larner College of Medicine, Burlington, VT, United States; Department of Molecular Physiology and Biophysics, University of Vermont Larner College of Medicine, Burlington, VT, United States., Files DC; Department of Internal Medicine, Section on Pulmonary, Critical Care, Allergy, and Immunologic Diseases, Wake Forest School of Medicine, Winston-Salem, NC, United States.
Jazyk: angličtina
Zdroj: Journal of immunological methods [J Immunol Methods] 2022 Oct; Vol. 509, pp. 113329. Date of Electronic Publication: 2022 Aug 03.
DOI: 10.1016/j.jim.2022.113329
Abstrakt: Macrophages are important mediators of skeletal muscle function in both healthy and diseased states. In vivo specific depletion of macrophages provides an experimental method to understand physiological and pathophysiological effects of macrophages. Systemic depletion of macrophages can deplete skeletal muscle macrophages but also alters systemic inflammatory responses and metabolism, which confounds the muscle specific effects of macrophage depletion. The primary aim of this manuscript is to evaluate two methods of murine intramuscular macrophage depletion in an acute lung injury-associated indirect skeletal muscle wasting mouse model. Adult C57BL/6 (WT) and Macrophage Fas-Induced Apoptosis (MaFIA, C57BL/6-Tg) mice received clodronate liposomes or the dimerization drug AP20187 through intramuscular injection of the tibialis anterior muscle compartment, respectively. Vehicle control was injected in the contralateral muscle. We demonstrate intramuscular AP20187 in the MaFIA mouse depletes macrophages but causes an infiltration of CD45 intermediate neutrophils. In contrast, intramuscular clodronate liposomes successfully depletes macrophages without an associated increase in CD45 intermediate cells. In conclusion, intramuscular clodronate is effective for selective depletion of muscle macrophages without eliciting acute inflammation seen with AP20187 in MaFIA mice. This technique is an important tool to study the functional roles of macrophages in skeletal muscle.
Competing Interests: Declaration of Competing Interest The authors declare no conflicts of interest in this work.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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