Vertical transmission and pathological findings in the mother, the placenta and the offspring, during first and last thirds of gestation in a mouse model of congenital toxoplasmosis.

Autor: Vargas-Villavicencio JA; Laboratorio de Inmunología Experimental, Instituto Nacional de Pediatría, SSa. Av. Insurgentes Sur 3700-C. Col. Insurgentes Cuicuilco, C.P. 04530 Mexico City, Mexico., Cedillo-Peláez C; Laboratorio de Inmunología Experimental, Instituto Nacional de Pediatría, SSa. Av. Insurgentes Sur 3700-C. Col. Insurgentes Cuicuilco, C.P. 04530 Mexico City, Mexico., Aguilar-Orozco MI; Laboratorio de Inmunología Experimental, Instituto Nacional de Pediatría, SSa. Av. Insurgentes Sur 3700-C. Col. Insurgentes Cuicuilco, C.P. 04530 Mexico City, Mexico; Programa de Posgrado en Ciencias de la Producción y de la Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México. Av. Universidad 3000. Delegación Coyoacán, C.P. 04510 Mexico City, Mexico., Rico-Torres CP; Laboratorio de Inmunología Experimental, Instituto Nacional de Pediatría, SSa. Av. Insurgentes Sur 3700-C. Col. Insurgentes Cuicuilco, C.P. 04530 Mexico City, Mexico., Farfan-Morales JE; Departamento de Patología, Instituto Nacional de Pediatría, SSa. Av. Insurgentes Sur 3700-C. Col. Insurgentes Cuicuilco, C.P. 04530, Mexico City, Mexico., Correa D; Laboratorio de Inmunología Experimental, Instituto Nacional de Pediatría, SSa. Av. Insurgentes Sur 3700-C. Col. Insurgentes Cuicuilco, C.P. 04530 Mexico City, Mexico; Dirección de Investigación, Centro de Investigación en Ciencias de la Salud, CICSA-FCS, Universidad Anáhuac, Campus Norte México. Av Universidad Anáhuac 46, Lomas Anáhuac, Huixquilucan, Estado de México C.P. 52786, Mexico. Electronic address: dolores.correa@anahuac.mx.
Jazyk: angličtina
Zdroj: Parasitology international [Parasitol Int] 2022 Dec; Vol. 91, pp. 102640. Date of Electronic Publication: 2022 Aug 04.
DOI: 10.1016/j.parint.2022.102640
Abstrakt: We performed a study of congenital toxoplasmosis of the first and third gestation periods in mice, and determined its effects on the embryos/fetuses, the placentae and the maternal organs. We infected pregnant BALB/c mice by i.v. injection of 2.5--10.0 × 10 6 tachyzoites of the ME49 T. gondii strain and euthanized them 72 h later. The tissues were analyzed by histopathology, immunohistochemistry and parasite-specific qPCR. Infections with the lowest dose induced remarkably different changes in the two thirds: a) all doses diminished the number of products/litter, the lowest dose only by 14%; but most embryos still visible were degenerated in the case of the first period, while the fetuses of the last third were perfectly preserved; b) the transmission rate in the first third was relatively high, but with a very low parasite burden; c) with the lowest dose, strong vascular changes (congestion, thrombosis and hemorrhage) predominated in the placentas of the first period, while they were absent in the last third; d) necrosis caused by T. gondii to maternal organs was much stronger during the last gestation period than in the first. Our results suggest that the vascular alterations at the placenta of the first third of pregnancy prevent embryo from large parasite burden, but provoke its death by starvation. In the last gestation period, there was poor control of parasite dissemination to the placenta and the fetus, but there was greater capacity of the product to defend itself from T. gondii.
Competing Interests: Declaration of Competing Interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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