The enigmatic physiological roles of AhpCF, Gpx, Npr and Kat in peroxide stress response of Enterococcus faecium.

Autor: Wasselin V; Normandie Univ, UNICAEN U2RM-Stress and Virulence, Esplanade de la Paix, 14032 Caen, France. Electronic address: wasselin.v@gmail.com., Budin-Verneuil A; Normandie Univ, UNICAEN U2RM-Stress and Virulence, Esplanade de la Paix, 14032 Caen, France. Electronic address: aurelie.verneuil@unicaen.fr., Rincé I; Normandie Univ, UNICAEN U2RM-Stress and Virulence, Esplanade de la Paix, 14032 Caen, France. Electronic address: isabelle.rince@unicaen.fr., Léger L; Normandie Univ, UNICAEN U2RM-Stress and Virulence, Esplanade de la Paix, 14032 Caen, France. Electronic address: loic.leger@outlook.fr., Boukerb AM; Normandie Univ, LMSM EA4312-Microbiology Signals and Microenvironment, 27000, Evreux, France. Electronic address: amine.boukerb@univ-rouen.fr., Hartke A; Normandie Univ, UNICAEN U2RM-Stress and Virulence, Esplanade de la Paix, 14032 Caen, France. Electronic address: axel.hartke@unicaen.fr., Benachour A; Normandie Univ, UNICAEN U2RM-Stress and Virulence, Esplanade de la Paix, 14032 Caen, France. Electronic address: abdellah.benachour@unicaen.fr., Riboulet-Bisson E; Normandie Univ, UNICAEN U2RM-Stress and Virulence, Esplanade de la Paix, 14032 Caen, France. Electronic address: eliette.bisson@unicaen.fr.
Jazyk: angličtina
Zdroj: Research in microbiology [Res Microbiol] 2022 Oct-Dec; Vol. 173 (8), pp. 103982. Date of Electronic Publication: 2022 Aug 02.
DOI: 10.1016/j.resmic.2022.103982
Abstrakt: In this work, the physiological roles of the primary peroxide scavenging activities of Enterococcus faecium AUS0004 strain were analysed. This healthcare-associated pathogen harbours genes encoding putative NADH peroxidase (Npr), alkyl hydroperoxide reductase (AhpCF), glutathione peroxidase (Gpx) and manganese-dependent catalase (Mn-Kat). Gene expression analyses showed that npr and kat genes are especially and significantly induced in cells treated with hydrogen peroxide (H 2 O 2 ) and cumene hydroperoxide (CuOOH), which suggested an important function of these enzymes to protect E. faecium against peroxide stress. Mutants affected in one or several predicted anti-oxidative activities mentioned above showed that neither the peroxidases nor the catalase are implicated in the defence against peroxide challenges. However, our investigations allowed us to show that Npr is responsible for the degradation of approximately 45% of metabolically derived H 2 O 2 which avoids accumulation of the peroxide to lethal concentrations.
Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest.
(Copyright © 2022 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE