Effects of propofol and dexmedetomidine on carnitine metabolism in normal human bronchial epithelial cells.

Autor: Büyükfırat E; Department of Anesthesiology and Reanimation, Medical Faculty, Harran University, Turkey., Koyuncu I; Department of Medical Biochemistry, Medical Faculty, Harran University, Turkey., Karahan MA; Department of Anesthesiology and Reanimation, Medical Faculty, Harran University, Turkey., Gönel A; Department of Medical Biochemistry, Medical Faculty, Harran University, Turkey., Binici O; Department of Anesthesiology and Reanimation, Medical Faculty, Harran University, Turkey., Duran E; Department of Anesthesiology and Reanimation, Medical Faculty, Harran University, Turkey., Altay N; Department of Anesthesiology and Reanimation, Medical Faculty, Harran University, Turkey.
Jazyk: angličtina
Zdroj: Biotechnic & histochemistry : official publication of the Biological Stain Commission [Biotech Histochem] 2023 Jan; Vol. 98 (1), pp. 62-68. Date of Electronic Publication: 2022 Aug 05.
DOI: 10.1080/10520295.2022.2107239
Abstrakt: Propofol and dexmedetomidine (DEX) are widely used for anesthesia and sedation. We investigated the effects of propofol and DEX separately and in combination on the metabolic profile of carnitine in cultured normal human bronchial epithelial cells (BEAS-2B). Cells of the propofol group were cultured with 2 µg/ml propofol in RPMI-1640 medium. Cells of the DEX group were cultured with 0.2 ng/m DEX in RPMI-1640 medium. Cells of the propofol + DEX group were cultured with 2 μg/ml propofol + 0.2 ng/ml DEX in RPMI-1640 medium. The control group was untreated. Cells were incubated for 3 h following treatments. The effects of the drugs on cell viability were assessed using the MTT method and by microscopic examination following staining with acridine orange/ethidium bromide. The effects of drugs on carnitine, acetyl carnitine and 25 acylcarnitine derivative profiles were analyzed using liquid chromatography-tandem mass spectrophotometry. Neither propofol nor DEX affected cell viability. Administration of propofol, DEX or propofol + DEX to BEAS-2B cells caused no significant change in the concentrations of carnitine and acylcarnitine derivatives compared to the control group. We found that propofol and DEX exhibit no negative effects on the carnitine metabolism by BEAS-2B cells in vitro at clinically relevant concentrations. Our findings establish a baseline for clinical studies of the effects of propofol and DEX on carnitine metabolism.
Databáze: MEDLINE
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