High-fat diet affects autophagy and mitochondrial compartment in rat Harderian gland.

Autor: Chieffi Baccari G; Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Università degli studi della Campania 'Luigi Vanvitelli', Caserta, Italy., Falvo S; Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Università degli studi della Campania 'Luigi Vanvitelli', Caserta, Italy., Di Fiore MM; Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Università degli studi della Campania 'Luigi Vanvitelli', Caserta, Italy., Cioffi F; Dipartimento di Scienze e Tecnologie, Università degli studi del Sannio, Benevento, Italy., Giacco A; Dipartimento di Scienze e Tecnologie, Università degli studi del Sannio, Benevento, Italy., Santillo A; Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Università degli studi della Campania 'Luigi Vanvitelli', Caserta, Italy.
Jazyk: angličtina
Zdroj: Journal of experimental zoology. Part A, Ecological and integrative physiology [J Exp Zool A Ecol Integr Physiol] 2022 Dec; Vol. 337 (9-10), pp. 1025-1038. Date of Electronic Publication: 2022 Aug 04.
DOI: 10.1002/jez.2646
Abstrakt: The Harderian gland (HG) of Rattus norvegicus is an orbital gland secreting lipids that accumulate in excess under condition of increased lipid metabolism. To study the response elicitated by lipid overload in rat HG, we housed the animals in thermoneutral conditions (28-30°C) in association to high fat diet (HFD). In HFD rats alterated blood lipid levels result in lipid accumulation in HG as demonstrated by the increased gland weight and histochemical/ultrastructural analyses. The HFD-caused oxidative stress forces the gland to trigger antioxidant defense mechanisms and autophagic process, such as lipophagy and mitophagy. Induction of mitochondrial DNA (mtDNA) damage and repair was stronger in HFD-rat HGs. An increase in marker expression levels of mitochondrial biogenesis, fission, and fusion occurred to counteract mtDNA copy number reduction and mitophagy. Therefore, the results demonstrate that rat HG activates autophagy as survival strategy under conditions of increased lipid metabolism and suggest a key role for mitophagy and membrane dynamics in the mitochondrial adaptive response to HFD.
(© 2022 Wiley Periodicals LLC.)
Databáze: MEDLINE
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