Adaptation to short-term extreme fat consumption alters intestinal lipid handling in male and female mice.

Autor: Morrow NM; The University of Ottawa Heart Institute, 40 Ruskin Street, H-3229A, Ottawa KIY 4W7, Ontario, Canada; Department of Biochemistry, Microbiology and Immunology, The University of Ottawa, Faculty of Medicine, 451 Smyth Rd, Ottawa, ON K1H 8L1, Canada., Locatelli CAA; The University of Ottawa Heart Institute, 40 Ruskin Street, H-3229A, Ottawa KIY 4W7, Ontario, Canada; Department of Biochemistry, Microbiology and Immunology, The University of Ottawa, Faculty of Medicine, 451 Smyth Rd, Ottawa, ON K1H 8L1, Canada., Trzaskalski NA; The University of Ottawa Heart Institute, 40 Ruskin Street, H-3229A, Ottawa KIY 4W7, Ontario, Canada; Department of Biochemistry, Microbiology and Immunology, The University of Ottawa, Faculty of Medicine, 451 Smyth Rd, Ottawa, ON K1H 8L1, Canada., Klein CT; The University of Ottawa Heart Institute, 40 Ruskin Street, H-3229A, Ottawa KIY 4W7, Ontario, Canada; Translational and Molecular Medicine, The University of Ottawa, Faculty of Medicine, 451 Smyth Rd, Ottawa, ON K1H 8L1, Canada., Hanson AA; The University of Ottawa Heart Institute, 40 Ruskin Street, H-3229A, Ottawa KIY 4W7, Ontario, Canada; Department of Biochemistry, Microbiology and Immunology, The University of Ottawa, Faculty of Medicine, 451 Smyth Rd, Ottawa, ON K1H 8L1, Canada., Alhadi H; The University of Ottawa Heart Institute, 40 Ruskin Street, H-3229A, Ottawa KIY 4W7, Ontario, Canada; Translational and Molecular Medicine, The University of Ottawa, Faculty of Medicine, 451 Smyth Rd, Ottawa, ON K1H 8L1, Canada., Tripathi I; The University of Ottawa Heart Institute, 40 Ruskin Street, H-3229A, Ottawa KIY 4W7, Ontario, Canada; Translational and Molecular Medicine, The University of Ottawa, Faculty of Medicine, 451 Smyth Rd, Ottawa, ON K1H 8L1, Canada., Clément AC; The University of Ottawa Heart Institute, 40 Ruskin Street, H-3229A, Ottawa KIY 4W7, Ontario, Canada; Department of Biochemistry, Microbiology and Immunology, The University of Ottawa, Faculty of Medicine, 451 Smyth Rd, Ottawa, ON K1H 8L1, Canada; Centre for Infection, Immunity and Inflammation, The University of Ottawa, 451 Smyth Rd, Ottawa, K1H 8M5, ON, Canada., Imran S; The University of Ottawa Heart Institute, 40 Ruskin Street, H-3229A, Ottawa KIY 4W7, Ontario, Canada; Department of Biochemistry, Microbiology and Immunology, The University of Ottawa, Faculty of Medicine, 451 Smyth Rd, Ottawa, ON K1H 8L1, Canada., Lorenzen-Schmidt I; The University of Ottawa Heart Institute, 40 Ruskin Street, H-3229A, Ottawa KIY 4W7, Ontario, Canada., Mulvihill EE; The University of Ottawa Heart Institute, 40 Ruskin Street, H-3229A, Ottawa KIY 4W7, Ontario, Canada; Department of Biochemistry, Microbiology and Immunology, The University of Ottawa, Faculty of Medicine, 451 Smyth Rd, Ottawa, ON K1H 8L1, Canada; Montreal Diabetes Research Centre, CRCHUM-Pavillion R, 900 Saint-Denis-Room R08.414, Montreal, H2X 0A9, QC, Canada; Centre for Infection, Immunity and Inflammation, The University of Ottawa, 451 Smyth Rd, Ottawa, K1H 8M5, ON, Canada. Electronic address: emulvihi@uottawa.ca.
Jazyk: angličtina
Zdroj: Biochimica et biophysica acta. Molecular and cell biology of lipids [Biochim Biophys Acta Mol Cell Biol Lipids] 2022 Nov; Vol. 1867 (11), pp. 159208. Date of Electronic Publication: 2022 Aug 01.
DOI: 10.1016/j.bbalip.2022.159208
Abstrakt: The small intestine is a highly adaptable organ serving as both a barrier to the external environment and a conduit for nutrient absorption. Enterocytes package dietary triglycerides (TG) into chylomicrons for transport into circulation; the remaining TGs are stored in cytosolic lipid droplets (CLDs). The current study aimed to characterize the impact of diet composition on intestinal lipid handling in male and female wild-type mice. Mice were continued on their grain-based diet (GBD) and switched to either a high-fat, high cholesterol Western-style diet (WD) or a ketogenic diet (KD) for 3 or 5 weeks. KD-fed mice displayed significantly higher plasma TG levels in response to an olive oil gavage than WD- and GBD-fed mice; TG levels were ~2-fold higher in male KD-fed mice than female KD-fed mice. Poloxamer-407 experiments revealed enhanced intestinal-TG secretion rates in male mice fed a KD upon olive oil gavage, whereas secretion rates were unchanged in female mice. Surprisingly, jejunal CLD size and TG mass after oil gavage were similar among the groups. At fasting, TG mass was significantly higher in the jejunum of male KD-fed mice and the duodenum of female KD-fed mice, providing increased substrate for chylomicron formation. In addition to greater fasting intestinal TG stores, KD-fed male mice displayed longer small intestinal lengths, while female mice displayed markedly longer jejunal villi lengths. After 5 weeks of diet, 12 h fasting-2 h refeeding experiments revealed jejunal TG levels were similar between diet groups in male mice; however, in female mice, jejunal TG mass was significantly higher in KD-fed mice compared to GBD- and WD-fed mice. These experiments reveal that KD feeding promotes distinct morphological and functional changes to the murine small intestine compared to the WD diet. Moreover, changes to intestinal lipid handling in response to carbohydrate and protein restriction manifest differently in male and female mice.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Erin Mulvihill reports a relationship with Merck Canada Inc. that includes: funding grants.
(Copyright © 2022. Published by Elsevier B.V.)
Databáze: MEDLINE
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