Fetal loss in pregnant rhesus macaques infected with high-dose African-lineage Zika virus.

Autor: Raasch LE; Department of Pathology and Laboratory Medicine, UW Madison, Madison, Wisconsin, United States of America., Yamamoto K; Department of Pathology and Laboratory Medicine, UW Madison, Madison, Wisconsin, United States of America., Newman CM; Department of Pathology and Laboratory Medicine, UW Madison, Madison, Wisconsin, United States of America., Rosinski JR; Department of Pathology and Laboratory Medicine, UW Madison, Madison, Wisconsin, United States of America., Shepherd PM; Department of Pathology and Laboratory Medicine, UW Madison, Madison, Wisconsin, United States of America., Razo E; Department of Pediatrics, UW Madison, Madison, Wisconsin, United States of America., Crooks CM; Department of Pathobiological Sciences, UW Madison, Madison, Wisconsin, United States of America., Bliss MI; Wisconsin National Primate Research Center, UW Madison, Madison, Wisconsin, United States of America., Breitbach ME; Department of Pathology and Laboratory Medicine, UW Madison, Madison, Wisconsin, United States of America., Sneed EL; Wisconsin National Primate Research Center, UW Madison, Madison, Wisconsin, United States of America., Weiler AM; Wisconsin National Primate Research Center, UW Madison, Madison, Wisconsin, United States of America., Zeng X; United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, United States of America., Noguchi KK; Department of Psychiatry, Washington University School of Medicine, Saint Louis, Missouri, United States of America., Morgan TK; Department of Pathology, Oregon Health and Science University, Portland, Oregon, United States of America.; Department of Obstetrics and Gynecology, Oregon Health and Science University, Portland, Oregon, United States of America., Fuhler NA; Department of Psychiatry, Washington University School of Medicine, Saint Louis, Missouri, United States of America., Bohm EK; Department of Veterinary and Biomedical Sciences, University of Minnesota, Minneapolis, Minnesota, United States of America., Alberts AJ; Department of Pathology and Laboratory Medicine, UW Madison, Madison, Wisconsin, United States of America., Havlicek SJ; Department of Pathology and Laboratory Medicine, UW Madison, Madison, Wisconsin, United States of America., Kabakov S; Department of Kinesiology Occupational Therapy Program, University of Wisconsin Madison, Madison, Wisconsin, United States of America., Mitzey AM; Department of Comparative Biosciences, UW Madison, Madison, Wisconsin, United States of America., Antony KM; Department of Obstetrics and Gynecology, UW Madison, Madison, Wisconsin, United States of America., Ausderau KK; Department of Kinesiology Occupational Therapy Program, University of Wisconsin Madison, Madison, Wisconsin, United States of America.; Waisman Center, UW Madison, Madison, Wisconsin, United States of America., Mejia A; Wisconsin National Primate Research Center, UW Madison, Madison, Wisconsin, United States of America., Basu P; Wisconsin National Primate Research Center, UW Madison, Madison, Wisconsin, United States of America., Simmons HA; Wisconsin National Primate Research Center, UW Madison, Madison, Wisconsin, United States of America., Eickhoff JC; Department of Biostatistics and Medical Informatics, UW Madison, Madison, Wisconsin, United States of America., Aliota MT; Department of Veterinary and Biomedical Sciences, University of Minnesota, Minneapolis, Minnesota, United States of America., Mohr EL; Department of Pediatrics, UW Madison, Madison, Wisconsin, United States of America., Friedrich TC; Department of Pathobiological Sciences, UW Madison, Madison, Wisconsin, United States of America.; Wisconsin National Primate Research Center, UW Madison, Madison, Wisconsin, United States of America., Golos TG; Wisconsin National Primate Research Center, UW Madison, Madison, Wisconsin, United States of America.; Department of Veterinary and Biomedical Sciences, University of Minnesota, Minneapolis, Minnesota, United States of America.; Department of Kinesiology Occupational Therapy Program, University of Wisconsin Madison, Madison, Wisconsin, United States of America., O'Connor DH; Department of Pathology and Laboratory Medicine, UW Madison, Madison, Wisconsin, United States of America.; Wisconsin National Primate Research Center, UW Madison, Madison, Wisconsin, United States of America., Dudley DM; Department of Pathology and Laboratory Medicine, UW Madison, Madison, Wisconsin, United States of America.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2022 Aug 04; Vol. 16 (8), pp. e0010623. Date of Electronic Publication: 2022 Aug 04 (Print Publication: 2022).
DOI: 10.1371/journal.pntd.0010623
Abstrakt: Countermeasures against Zika virus (ZIKV), including vaccines, are frequently tested in nonhuman primates (NHP). Macaque models are important for understanding how ZIKV infections impact human pregnancy due to similarities in placental development. The lack of consistent adverse pregnancy outcomes in ZIKV-affected pregnancies poses a challenge in macaque studies where group sizes are often small (4-8 animals). Studies in small animal models suggest that African-lineage Zika viruses can cause more frequent and severe fetal outcomes. No adverse outcomes were observed in macaques exposed to 1x104 PFU (low dose) of African-lineage ZIKV at gestational day (GD) 45. Here, we exposed eight pregnant rhesus macaques to 1x108 PFU (high dose) of African-lineage ZIKV at GD 45 to test the hypothesis that adverse pregnancy outcomes are dose-dependent. Three of eight pregnancies ended prematurely with fetal death. ZIKV was detected in both fetal and placental tissues from all cases of early fetal loss. Further refinements of this exposure system (e.g., varying the dose and timing of infection) could lead to an even more consistent, unambiguous fetal loss phenotype for assessing ZIKV countermeasures in pregnancy. These data demonstrate that high-dose exposure to African-lineage ZIKV causes pregnancy loss in macaques and also suggest that ZIKV-induced first trimester pregnancy loss could be strain-specific.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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