Intermittent bulk release of human cytomegalovirus.

Autor: Flomm FJ; Centre for Structural Systems Biology, Hamburg, Germany.; Hannover Medical School, Institute of Virology, Hannover, Germany.; Cluster of Excellence RESIST (EXC 2155), Hannover Medical School, Hannover, Germany.; Leibniz-Institute of Virology (LIV), Hamburg, Germany., Soh TK; Centre for Structural Systems Biology, Hamburg, Germany.; Hannover Medical School, Institute of Virology, Hannover, Germany.; Cluster of Excellence RESIST (EXC 2155), Hannover Medical School, Hannover, Germany.; Leibniz-Institute of Virology (LIV), Hamburg, Germany., Schneider C; Leibniz-Institute of Virology (LIV), Hamburg, Germany., Wedemann L; Centre for Structural Systems Biology, Hamburg, Germany.; Hannover Medical School, Institute of Virology, Hannover, Germany.; Cluster of Excellence RESIST (EXC 2155), Hannover Medical School, Hannover, Germany.; Leibniz-Institute of Virology (LIV), Hamburg, Germany., Britt HM; Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London, United Kingdom., Thalassinos K; Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London, United Kingdom.; Institute of Structural and Molecular Biology, Birkbeck College, University of London, London, United Kingdom., Pfitzner S; Leibniz-Institute of Virology (LIV), Hamburg, Germany., Reimer R; Leibniz-Institute of Virology (LIV), Hamburg, Germany., Grünewald K; Centre for Structural Systems Biology, Hamburg, Germany.; Cluster of Excellence RESIST (EXC 2155), Hannover Medical School, Hannover, Germany.; Leibniz-Institute of Virology (LIV), Hamburg, Germany.; University of Hamburg, Department of Chemistry, Hamburg, Germany., Bosse JB; Centre for Structural Systems Biology, Hamburg, Germany.; Hannover Medical School, Institute of Virology, Hannover, Germany.; Cluster of Excellence RESIST (EXC 2155), Hannover Medical School, Hannover, Germany.; Leibniz-Institute of Virology (LIV), Hamburg, Germany.
Jazyk: angličtina
Zdroj: PLoS pathogens [PLoS Pathog] 2022 Aug 04; Vol. 18 (8), pp. e1010575. Date of Electronic Publication: 2022 Aug 04 (Print Publication: 2022).
DOI: 10.1371/journal.ppat.1010575
Abstrakt: Human Cytomegalovirus (HCMV) can infect a variety of cell types by using virions of varying glycoprotein compositions. It is still unclear how this diversity is generated, but spatio-temporally separated envelopment and egress pathways might play a role. So far, one egress pathway has been described in which HCMV particles are individually enveloped into small vesicles and are subsequently exocytosed continuously. However, some studies have also found enveloped virus particles inside multivesicular structures but could not link them to productive egress or degradation pathways. We used a novel 3D-CLEM workflow allowing us to investigate these structures in HCMV morphogenesis and egress at high spatio-temporal resolution. We found that multiple envelopment events occurred at individual vesicles leading to multiviral bodies (MViBs), which subsequently traversed the cytoplasm to release virions as intermittent bulk pulses at the plasma membrane to form extracellular virus accumulations (EVAs). Our data support the existence of a novel bona fide HCMV egress pathway, which opens the gate to evaluate divergent egress pathways in generating virion diversity.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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