Combined small and large magnetic nanoparticle extraction and concentration from biofluids for non-toxic detection of biomarkers.

Autor:	Lapchuk AS; Department of Optical Engineering, Institute for Information Recording of NAS of Ukraine 03113 Kyiv Ukraine alapchuk@yahoo.com., Gorbov IV; Department of Optical Engineering, Institute for Information Recording of NAS of Ukraine 03113 Kyiv Ukraine alapchuk@yahoo.com., Prygun AV; Department of Optical Engineering, Institute for Information Recording of NAS of Ukraine 03113 Kyiv Ukraine alapchuk@yahoo.com., Balagura IV; Department of Optical Engineering, Institute for Information Recording of NAS of Ukraine 03113 Kyiv Ukraine alapchuk@yahoo.com., Morozov YM; Department of Optical Engineering, Institute for Information Recording of NAS of Ukraine 03113 Kyiv Ukraine alapchuk@yahoo.com.; Biosensor Technologies, AIT-Austrian Institute of Technology 3430 Tulln Austria Yevhenii.Morozov@ait.ac.at.
Jazyk:	angličtina
Zdroj:	Sensors & diagnostics [Sens Diagn] 2022 Jun 09; Vol. 1 (4), pp. 829-840. Date of Electronic Publication: 2022 Jun 09 (Print Publication: 2022).
DOI:	10.1039/d2sd00078d
Abstrakt:	We propose a novel non-toxic method of diagnostic biomarker extraction and concentration from biofluids. The method is based on the usage of (1) magnetic nanoparticles of a few nanometres in size bearing molecular traps for biomarkers on their surface and (2) additional larger (several tens of nanometres) magnetic nanoparticles for catching smaller magnetic nanoparticles in a strong magnetic field gradient with their consequent concentration into the detection area. It is shown that the interference of an external permanent gradient magnetic field with the magnetic field of large magnetic nanoparticles allows one to catch small magnetic nanoparticles from their trajectories in a fluid at a distance around ten radii of the large nanoparticles. Theoretical analysis and mathematical simulation show the validity of the proposed non-toxic method for fast and robust biomarker extraction and concentration for increasing the sensitivity of biomarker detection. We believe that the results presented herein can serve as a starting point in the development of a new subclass of biosensors and a human body diagnostic approach with enhanced sensitivity and selectivity. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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