Post-treatment with metformin improves random skin flap survival through promoting angiogenesis in rats.

Autor:	Karimipour M; Department of Anatomical Sciences, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran., Farjah GH; Department of Anatomical Sciences, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.; Neurosciences Research Center, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran., Hassanzadeh M; Department of Anatomical Sciences, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran., Zirak Javanmard M; Department of Anatomical Sciences, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Jazyk:	angličtina
Zdroj:	Veterinary research forum : an international quarterly journal [Vet Res Forum] 2022 Jun; Vol. 13 (2), pp. 233-239. Date of Electronic Publication: 2022 Jun 15.
DOI:	10.30466/vrf.2020.124592.2917
Abstrakt:	Skin flap necrosis has been remained as an unsolved problem in plastic and reconstructive surgeries. Here, we explored the effects of metformin post-treatment on random skin flap survival in rats. An 8.00 × 2.00 cm dorsal skin flap was created in 24 rats and they were then divided into three groups (n = 8) including Control, metformin (Met) 50.00 mg kg -1 and Met 100 mg kg -1 . All animals were administrated orally until seven days after flap surgery. Flap survival, the number of blood vessels and mast cells in the flap tissues were analyzed. Vascular endothelial growth factor (VEGF) expression levels in flap tissues was also determined using immunohistochemical methods. The percentage of survival area in Met 50.00 mg kg -1 and Met 100 mg kg -1 groups were significantly higher compared to control. The blood vessel density and the VEGF positive cells in the viable areas of flaps showed a significant increase in Met 50.00 mg kg -1 group compared to control group. The results of this study suggested that treatment with metformin, especially with low dose following skin flap surgery was effective in improving the flap survival and increasing the neovascularization in the flaps tissues of rats. Competing Interests: The authors declared that no competing interest exists. (© 2022 Urmia University. All rights reserved.)
Databáze:	MEDLINE
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