Bi-layered living cellular construct resulted in greater healing in an alloxan-induced diabetic porcine model.

Autor: Avery JT; Department of Research & Development, Organogenesis Inc., Canton, Massachusetts, USA., Qiao J; Editas Medicine, Cambridge, Massachusetts, USA., Medeiros E; Department of Quality Control, Organogenesis Inc., Canton, Massachusetts, USA., Bollenbach TJ; Advanced Regenerative Manufacturing Institute, Manchester, New Hampshire, USA., Kimmerling KA; Department of Research & Development, Organogenesis Inc., Canton, Massachusetts, USA., Mowry KC; Department of Research & Development, Organogenesis Inc., Canton, Massachusetts, USA.
Jazyk: angličtina
Zdroj: International wound journal [Int Wound J] 2023 Feb; Vol. 20 (2), pp. 403-412. Date of Electronic Publication: 2022 Aug 02.
DOI: 10.1111/iwj.13889
Abstrakt: Tissue-engineered skin constructs, including bi-layered living cellular constructs (BLCC) used in the treatment of chronic wounds, are structurally/functionally complex. While some work has been performed to understand their mechanisms, the totality of how BLCC may function in wound healing remains unknown. To this end, we have developed a delayed wound healing model to test BLCC cellular and molecular mechanisms of action. Diabetes was chemically-induced using alloxan in Yucatan miniature pigs, and full-thickness wounds were generated on their dorsum. These wounds were either allowed to heal by secondary intention alone (control) or treated with a single or multiple treatments of a porcine autologous BLCC. Results indicated a single treatment with porcine BLCC resulted in statistically significant wound healing at day 17, while four treatments resulted in statistically significant healing on days 10, 13, and 17 compared to control. Statistically accelerated wound closure was driven by re-epithelialisation rather than contraction or granulation. This porcine diabetic model and the use of a porcine BLCC allowed evaluation of healing responses in vivo without the complications typically seen with either xenogenic responses of human/animal systems or the use of immune compromised animals, expanding the knowledge base around how BLCC may impact chronic wounds.
(© 2022 Organogenesis. International Wound Journal published by Medicalhelplines.com Inc (3M) and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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