| Autor: |
Yıldırım M; Department of Pharmacy Services, Vocational School of Health Services, Tarsus University, Mersin, Turkey.; Department of Biopharmaceutics and Pharmaceutical Technology, Pharmacy Faculty, Saarland University, Saarbrücken, Germany., Erşatır M; Department of Chemistry, Arts and Science Faculty, Cukurova University, Adana, Turkey., Değirmenci U; Department of Biochemistry, Pharmacy Faculty, Harran University, Sanlıurfa, Turkey., Yigin A; Department of Genetic, Faculty of Veterinary, Harran University, Sanlıurfa, Turkey., Unal MB; Department of Histology, Faculty of Veterinary, Harran University, Sanlıurfa, Turkey., Guldur ME; Department of Pathology, Faculty of Medicine, Harran University, Sanlıurfa, Turkey., Demirkol O; Department of Chemistry, Arts and Science Faculty, Cukurova University, Adana, Turkey., Giray ES; Department of Chemistry, Arts and Science Faculty, Cukurova University, Adana, Turkey. |
| Abstrakt: |
In this study, the protective effects of Ruta chalepensis L . extracts on the extent of tissue damage in gentamicin-induced nephrotoxicity have been investigated. Ruta chalepensis L . extracts were prepared by subcritical water and ultrasound-assisted organic solvent extraction methods. Protective activity of Ruta chalepensis L . extracts on Gentamicin-induced nephrotoxicity is investigated by apoptotic, DNA damage, oxidative stress markers and evaluating histopathological in kidney tissue of mice. Gentamicin significantly increased Caspase-3 and -8 activities, NO levels, serum creatinine and BUN, while 8-OHdG and MDA levels were significantly decreased with Ruta chalepensis L. extract treatment. In addition, Ruta chalepensis L . extracts treatment significantly increased CAT and SOD activities. Histopathological alterations in Gentamicin group were significantly diminished by application of Ruta chalepensis L . extracts. These results suggest that treatment with Ruta chalepensis L . extracts may ameliorate renal dysfunction and structural damage through the reduction of oxidative stress and apoptosis in the kidney. |
