| Autor: |
Noell K; Departments of Pediatrics and Medicine, Center for Translational Research in Infection and Inflammation, and., Dai G; Departments of Pediatrics and Medicine, Center for Translational Research in Infection and Inflammation, and., Pungan D; Departments of Pediatrics and Medicine, Center for Translational Research in Infection and Inflammation, and., Ebacher A; Departments of Pediatrics and Medicine, Center for Translational Research in Infection and Inflammation, and., McCombs JE; Departments of Pediatrics and Medicine, Center for Translational Research in Infection and Inflammation, and., Landry SJ; Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, New Orleans, Louisiana, USA., Kolls JK; Departments of Pediatrics and Medicine, Center for Translational Research in Infection and Inflammation, and. |
| Abstrakt: |
Pneumocystis is the most common fungal pulmonary infection in children under the age of 5 years. In children with primary immunodeficiency, Pneumocystis often presents at 3-6 months of age, a time period that coincides with the nadir of maternal IgG and when IgM is the dominant Ig isotype. Because B cells are the dominant antigen-presenting cells for Pneumocystis, we hypothesized the presence of fungal-specific IgMs in humans and mice and that these IgM specificities would predict T cell antigens. We detected fungal-specific IgMs in human and mouse sera and utilized immunoprecipitation to determine whether any antigens were similar across donors. We then assessed T cell responses to these antigens and found anti-Pneumocystis IgM in WT mice, Aicda-/- mice, and in human cord blood. Immunoprecipitation of Pneumocystis murina with human cord blood identified shared antigens among these donors. Using class II MHC binding prediction, we designed peptides with these antigens and identified robust peptide-specific lung T cell responses after P. murina infection. After mice were immunized with 2 of the antigens, adoptive transfer of vaccine-elicited CD4+ T cells showed effector activity, suggesting that these antigens contain protective Pneumocystis epitopes. These data support the notion that germline-encoded IgM B cell receptors are critical in antigen presentation and T cell priming in early Pneumocystis infection. |
