Silver nanoparticles associated with a polyphosphate and fluoride enhance the prevention of enamel demineralization and impact on dual-biofilm adhesion.

Autor: Mendes-Gouvêa CC; Graduate Program of Dental Science, School of Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo 16015-050, Brazil., Danelon M; School of Dentistry, University of Ribeirão Preto - UNAERP, Ribeirão Preto, São Paulo 14096-900, Brazil; Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo 16015-050, Brazil., Vieira APM; Graduate Program of Dental Science, School of Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo 16015-050, Brazil., do Amaral JG; Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo 16015-050, Brazil., de Souza Neto FN; Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo 16015-050, Brazil., Gorup LF; Institute of Chemistry, Federal University of Alfenas, Alfenas, Minas Gerais, 37130-001, Brazil., Camargo ER; Department of Chemistry, Federal University of São Carlos, São Carlos, São Paulo 13565-905, Brazil., Delbem ACB; Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo 16015-050, Brazil., Barbosa DB; Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araçatuba, São Paulo 16015-050, Brazil. Electronic address: debora.b.barbosa@unesp.br.
Jazyk: angličtina
Zdroj: Journal of dentistry [J Dent] 2022 Oct; Vol. 125, pp. 104245. Date of Electronic Publication: 2022 Jul 30.
DOI: 10.1016/j.jdent.2022.104245
Abstrakt: Objectives: The aim of this study were to produce a multifunctional nanocomposite combining silver nanoaparticles (Ag), sodium trimetaphosphate (TMP) and fluoride (F), to investigate its effect on dental enamel demineralization and on biofilms of Streptococcus mutans and Candida albicans.
Methods: Bovine enamel blocks were submitted to five pH cycles and treated 2x/day with 100 ppm F, 225 ppm F, 100 ppm F + 0.2%TMP or 100 ppm F + 0.2%TMP+10% Ag (100F/TMP/Ag). Next, surface hardness loss (%SH), integrated loss of subsurface hardness (ΔKHN), enamel fluoride (F) and calcium (Ca) concentration were determined. Biofilms from single and dual species of S. mutans and C. albicans were treated with 100F/TMP/Ag, Ag or chlorhexidine gluconate for 24 h. The antibiofilm effect was evaluated by colony-forming unit counting and Scanning Electron Microscopy.
Results: The nanocomposite reduced 43.0% of %SH and was similar with samples treated with 225F, 100F/TMP and 100/TMP/Ag. The attribute of F and/or TMP in reducing ΔKHN in 5-20 μm was not affected by the addiction of Ag (110F = 225F = 100F/TMP = 100F/TMP/Ag > Negative Control). Further, 100F/TMP/Ag strongly reduced viable cells of S. mutans in dual biofilms (∼5 log 10 cm 2 ) and structurally affected the biofilms.
Conclusion: The 100F/TMP/F promoted a protective effect against enamel demineralization and was able to significantly inhibit the growth of biofilms of S. mutans and C. albicans.
Clinical Significance: The focus on prevention and non-invasive dental treatment is the most effective and least costly way to improve the population's oral health conditions. We present a nanocomposite for a multiple approach in prevention of caries.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022. Published by Elsevier Ltd.)
Databáze: MEDLINE
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