| Autor: |
Chen YX; Advanced Science Research Center, Neuroscience Initiative, the City University of New York, Graduate Center New York; Macaulay Honors College, CUNY; Graduate School of Public Health and Health Policy, The City University of New York., Veerasammy K; Advanced Science Research Center, Neuroscience Initiative, the City University of New York, Graduate Center New York; Advanced Science Research Center, Structural Biology Initiative, the City University of New York, Graduate Center., Yin J; National Institute of Neurological Disorders and Stroke, National Institutes of Health., Choetso T; Advanced Science Research Center, Neuroscience Initiative, the City University of New York, Graduate Center New York; The City College of New York, CUNY., Zhong T; Princeton University., Choudhury MA; Advanced Science Research Center, Neuroscience Initiative, the City University of New York, Graduate Center New York; The City College of New York, CUNY; Macaulay Honors College, CUNY., Weng C; Advanced Science Research Center, Neuroscience Initiative, the City University of New York, Graduate Center New York; The City College of New York, CUNY; Macaulay Honors College, CUNY., Xu E; Ardrey Kell High School., Hein MA; The Borough of Manhattan Community College, CUNY; Gallatin School of Individualized Study, New York University., Abzalimov R; Advanced Science Research Center, Structural Biology Initiative, the City University of New York, Graduate Center; rabzalimov@gc.cuny.edu., He Y; Advanced Science Research Center, Neuroscience Initiative, the City University of New York, Graduate Center New York; yhe1@gc.cuny.edu. |
| Abstrakt: |
Lipid profiling, or lipidomics, is a well-established technique used to study the entire lipid content of a cell or tissue. Information acquired from lipidomics is valuable in studying the pathways involved in development, disease, and cellular metabolism. Many tools and instrumentations have aided lipidomics projects, most notably various combinations of mass spectrometry and liquid chromatography techniques. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) has recently emerged as a powerful imaging technique that complements conventional approaches. This novel technique provides unique information on the spatial distribution of lipids within tissue compartments, which was previously unattainable without the use of excessive modifications. The sample preparation of the MALDI MSI approach is critical and, therefore, is the focus of this paper. This paper presents a rapid lipid analysis of a large number of Drosophila brains embedded in optimal cutting temperature compound (OCT) to provide a detailed protocol for the preparation of small tissues for lipid analysis or metabolite and small molecule analysis through MALDI MSI. |
