[Expression analysis of miRNAs from the DLK1-DIO3 locus in CD4+ and CD14+ cells in patients with relapsing-remitting multiple sclerosis].
| Autor: | Baulina NM; Pirogov Russian National Research Medical University, Moscow, Russia., Kabaeva AR; Federal Center of Brain and Neurotechnologies, Moscow, Russia., Boyko AN; Pirogov Russian National Research Medical University, Moscow, Russia.; Federal Center of Brain and Neurotechnologies, Moscow, Russia., Favorova OO; Pirogov Russian National Research Medical University, Moscow, Russia. |
|---|---|
| Jazyk: | ruština |
| Zdroj: | Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova [Zh Nevrol Psikhiatr Im S S Korsakova] 2022; Vol. 122 (7. Vyp. 2), pp. 52-59. |
| DOI: | 10.17116/jnevro202212207252 |
| Abstrakt: | Objective: To analyze the expression of several previously unstudied miRNAs from the DLK1-DIO3 locus in peripheral blood mononuclear cells (PBMC) and in CD14 + and CD4 + cell subpopulations in patients with relapsing-remitting multiple sclerosis (RRMS) and healthy individuals. Material and Methods: MiRNA expression analysis was performed in PBMC, CD14 + , and CD4 + cells of 14 patients who did not take immunomodulatory drugs, and 14 individuals without autoimmune and inflammatory diseases by reverse transcription followed by real-time PCR. Results: Expression levels of 10 miRNAs selected based on different criteria were significantly higher in PBMC in men with RRMS compared to healthy men; in women, their expression did not change in a similar comparison. No mass unidirectional changes in the expression of these miRNAs in RRMS men were observed in CD14 + and CD4 + cells. High consistency in the expression of miRNA pairs was also not found. Conclusions: The analysis of PBMC showed the involvement of 10 more miRNAs encoded by genes from the DLK1-DIO3 locus in the development of RRMS. The main observed effect of the increase in the expression levels of these miRNAs in men with RRMS compared with healthy men is not achieved due to the contribution of CD14 + and CD4 + cells. This opens up possibilities for studying the involvement of miRNAs in other PBMC subpopulations in the pathological processes in RRMS. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|