A lipopolysaccharide-dependent phage infects a pseudomonad phytopathogen and can evolve to evade phage resistance.

Autor: Warring SL; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand., Malone LM; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand., Jayaraman J; The New Zealand Institute for Plant & Food Research Limited, Mt Albert, Auckland, New Zealand.; Bioprotection Aotearoa, Canterbury, New Zealand., Easingwood RA; Otago Centre for Electron Microscopy, University of Otago, Dunedin, New Zealand., Rigano LA; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.; Plant Health & Environment Laboratory, Biosecurity New Zealand, Ministry for Primary Industries, Auckland, New Zealand., Frampton RA; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.; The New Zealand Institute for Plant & Food Research Limited, Christchurch, New Zealand., Visnovsky SB; The New Zealand Institute for Plant & Food Research Limited, Christchurch, New Zealand., Addison SM; The New Zealand Institute for Plant & Food Research Limited, Christchurch, New Zealand., Hernandez L; The New Zealand Institute for Plant & Food Research Limited, Christchurch, New Zealand., Pitman AR; The New Zealand Institute for Plant & Food Research Limited, Christchurch, New Zealand.; Foundation for Arable Research (FAR), Templeton, Christchurch, New Zealand., Lopez Acedo E; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand., Kleffmann T; Centre for Protein Research, University of Otago, Dunedin, New Zealand., Templeton MD; The New Zealand Institute for Plant & Food Research Limited, Mt Albert, Auckland, New Zealand.; Bioprotection Aotearoa, Canterbury, New Zealand.; School of Biological Sciences, University of Auckland, Auckland, New Zealand., Bostina M; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.; Otago Centre for Electron Microscopy, University of Otago, Dunedin, New Zealand., Fineran PC; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.; Bioprotection Aotearoa, Canterbury, New Zealand.
Jazyk: angličtina
Zdroj: Environmental microbiology [Environ Microbiol] 2022 Oct; Vol. 24 (10), pp. 4834-4852. Date of Electronic Publication: 2022 Aug 01.
DOI: 10.1111/1462-2920.16106
Abstrakt: Bacterial pathogens are major causes of crop diseases, leading to significant production losses. For instance, kiwifruit canker, caused by the phytopathogen Pseudomonas syringae pv. actinidiae (Psa), has posed a global challenge to kiwifruit production. Treatment with copper and antibiotics, whilst initially effective, is leading to the rise of bacterial resistance, requiring new biocontrol approaches. Previously, we isolated a group of closely related Psa phages with biocontrol potential, which represent environmentally sustainable antimicrobials. However, their deployment as antimicrobials requires further insight into their properties and infection strategy. Here, we provide an in-depth examination of the genome of ΦPsa374-like phages and show that they use lipopolysaccharides (LPS) as their main receptor. Through proteomics and cryo-electron microscopy of ΦPsa374, we revealed the structural proteome and that this phage possess a T = 9 capsid triangulation, unusual for myoviruses. Furthermore, we show that ΦPsa374 phage resistance arises in planta through mutations in a glycosyltransferase involved in LPS synthesis. Lastly, through in vitro evolution experiments we showed that phage resistance is overcome by mutations in a tail fibre and structural protein of unknown function in ΦPsa374. This study provides new insight into the properties of ΦPsa374-like phages that informs their use as antimicrobials against Psa.
(© 2022 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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