Click-Chemistry-Based Biomimetic Ligands Efficiently Capture G-Quadruplexes In Vitro and Help Localize Them at DNA Damage Sites in Human Cells.

Autor:	Rota Sperti F; Institut de Chimie Moléculaire, ICMUB CNRS UMR 6302, UBFC Dijon, 21078 Dijon, France., Dupouy B; Institut de Chimie Moléculaire, ICMUB CNRS UMR 6302, UBFC Dijon, 21078 Dijon, France., Mitteaux J; Institut de Chimie Moléculaire, ICMUB CNRS UMR 6302, UBFC Dijon, 21078 Dijon, France., Pipier A; Institut de Chimie Moléculaire, ICMUB CNRS UMR 6302, UBFC Dijon, 21078 Dijon, France., Pirrotta M; Institut de Chimie Moléculaire, ICMUB CNRS UMR 6302, UBFC Dijon, 21078 Dijon, France., Chéron N; PASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS, 75005 Paris, France., Valverde IE; Institut de Chimie Moléculaire, ICMUB CNRS UMR 6302, UBFC Dijon, 21078 Dijon, France., Monchaud D; Institut de Chimie Moléculaire, ICMUB CNRS UMR 6302, UBFC Dijon, 21078 Dijon, France.
Jazyk:	angličtina
Zdroj:	JACS Au [JACS Au] 2022 Jun 17; Vol. 2 (7), pp. 1588-1595. Date of Electronic Publication: 2022 Jun 17 (Print Publication: 2022).
DOI:	10.1021/jacsau.2c00082
Abstrakt:	Interrogating G-quadruplex (G4) biology at its deepest roots in human cells relies on the design, synthesis, and use of ever smarter molecular tools. Here, we demonstrate the versatility of biomimetic G4 ligands referred to as TASQ (template assembled synthetic G-quartet) in which a biotin handle was incorporated for G4-focused chemical biology investigations. We have rethought the biotinylated TASQ design to make it readily chemically accessible via an efficient click-chemistry-based strategy. The resulting biotinylated, triazole-assembled TASQ, or BioTriazoTASQ, was thus shown to efficiently isolate both DNA and RNA G4s from solution by affinity purification protocols, for identification purposes. Its versatility was then further demonstrated by optical imaging that provided unique mechanistic insights into the actual strategic relevance of G4-targeting strategies, showing that ligand-stabilized G4 sites colocalize with and, thus, are responsible for DNA damage foci in human cells. Competing Interests: The authors declare no competing financial interest. (© 2022 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu