Attenuation of ovalbumin-induced inflammation and lung oxidative injury in asthmatic rats by Zingiber officinale extract: combined in silico and in vivo study on antioxidant potential, STAT6 and TNF- α pathways.

Autor: Jedli O; Laboratory of Histo-Embryology & Cytogenetics, Medicine Faculty of Sfax, University of Sfax, Magida Boulila Street, 3029 Sfax, Tunisia., Ben-Nasr H; Laboratory of Pharmacology, Medicine Faculty of Sfax, University of Sfax, Majida Boulila Street, 3029 Sfax, Tunisia., Zammel N; Laboratory of Histo-Embryology & Cytogenetics, Medicine Faculty of Sfax, University of Sfax, Magida Boulila Street, 3029 Sfax, Tunisia., Rebai T; Laboratory of Histo-Embryology & Cytogenetics, Medicine Faculty of Sfax, University of Sfax, Magida Boulila Street, 3029 Sfax, Tunisia., Saoudi M; Laboratory of Animal Ecophysiology, Department of Biology, Faculty of Sciences, University of Sfax, 3054 Sfax, Tunisia., Elkahoui S; Laboratory of General Biology, Department of Biology, University Ha'il, Ha'il, 81451 Saudi Arabia., Jamal A; Laboratory of General Biology, Department of Biology, University Ha'il, Ha'il, 81451 Saudi Arabia., Siddiqui AJ; Laboratory of General Biology, Department of Biology, University Ha'il, Ha'il, 81451 Saudi Arabia., Sulieman AE; Laboratory of General Biology, Department of Biology, University Ha'il, Ha'il, 81451 Saudi Arabia., Alreshidi MM; Laboratory of General Biology, Department of Biology, University Ha'il, Ha'il, 81451 Saudi Arabia., Naïli H; Laboratory of Solid State (LR11ES51), Faculty of Sciences, University of Sfax, 3000 Sfax, Tunisia., Badraoui R; Laboratory of General Biology, Department of Biology, University Ha'il, Ha'il, 81451 Saudi Arabia.; Section of Histology Cytology, Medicine Faculty of Tunis, El Manar University, 1007 La Rabta-Tunis, Tunisia.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2022 Sep; Vol. 12 (9), pp. 191. Date of Electronic Publication: 2022 Jul 26.
DOI: 10.1007/s13205-022-03249-5
Abstrakt: In the present study we focused on the anti-asthmatic and antioxidant effects of Zingiber officinalis roscoe L. (ZO) aqueous extract. This study includes 20 adult male rats, which were grouped into four; Group I: control group; Group II: asthmatic group (Ovalbumin sensitized/challenge model, Oval group); Group III: received ovalbumin sensitized/challenge associated a dose of 207 mg/kg body weight (BW) of ZO (Oval + D1 group); Group IV: received ovalbumin sensitized/challenge associated a dose of 414 mg/k BW of ZO (Oval + D2 group). After 21 days, blood and lung samples were collected for biochemical, hematological, and histopathological analyses. The ameliorative effect of ZO phytochemical compounds was also assessed by in silico approach on transducer and activator of transcription 6 (STAT6) and tumor necrosis factor- α (TNF- α ) receptors. The oxidative/antioxidative status was evaluated in the lung tissues. Our results show that ZO extract alleviated the ovalbumin-induced hematological and biochemical disruptions associated oxidative injury. In fact, white and red blood cells (WBC and RBC, respectively), aspartate aminotransaminase (ASAT), malondialdehyde (MDA), glutathione (GSH), and glutathione peroxidase (GPx) were significantly disrupted ( p  < 0.05) in Oval group and alleviated following ZO treatment. Besides, several histopathological features were outlined in lung tissues of Oval group. Interestingly, ZO was found to exert ameliorative effects on tissue level. In silico analyses, particularly the binding affinities, the number of H-bonds, the embedding distance and the molecular interactions of ZO phytochemical compounds with either STAT6 or TNF- α supported the in vivo results. These findings confirm the potential ethno-pharmacological effects of ZO against asthma and its associated complications.
Competing Interests: Conflict of interestThe authors declare that they have no conflicts of interest in the publication.
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Databáze: MEDLINE
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