Microglial Engulfment of Spines in the Ventral Zona Incerta Regulates Anxiety-Like Behaviors in a Mouse Model of Acute Pain.

Autor: Farzinpour Z; Department of Anesthesiology and Pain Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China., Liu A; Department of Physiology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China., Cao P; Department of Anesthesiology and Pain Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China., Mao Y; Department of Anesthesiology and Pain Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China., Zhang Z; Department of Anesthesiology and Pain Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China., Jin Y; Stroke Center and Department of Neurology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
Jazyk: angličtina
Zdroj: Frontiers in cellular neuroscience [Front Cell Neurosci] 2022 Jul 14; Vol. 16, pp. 898346. Date of Electronic Publication: 2022 Jul 14 (Print Publication: 2022).
DOI: 10.3389/fncel.2022.898346
Abstrakt: Although activation of microglial cells is critical in developing brain disorders, their role in anxiety-like behaviors in pain is still vague. This study indicates that alteration of microglia's neuronal spine engulfment capacity in ventral zona incerta (ZI V ) leads to significant pain and anxiety-like behaviors in mice 1-day post-injection of Complete Freud's Adjuvant (CFA1D). Performing whole-cell patch-clamp recordings in GABAergic neurons in the ZI V (ZI V GABA ) in brain slices, we observed decreased activity in ZIv GABA and reduced frequency of the miniature excitatory postsynaptic currents (mEPSCs) in ZI V GABA of CFA1D mice compared with the saline1D mice. Besides, chemogenetic activation of ZI V GABA significantly relieved pain and anxiety-like behaviors in CFA1D mice. Conversely, in naïve mice, chemogenetic inhibition of ZI V GABA induced pain and anxiety-like behaviors. Interestingly, we found changes in the density and morphology of ZI V Microglia and increased microglial engulfment of spines in ZI V of CFA1D mice. Furthermore, pain sensitization and anxiety-like behaviors were reversed when the ZI V Microglia of CFA1D-treated mice were chemically inhibited by intra-ZI V minocycline injection, accompanied by the recovery of decreased ZI V GABA excitability. Conclusively, our results provide novel insights that dysregulation of microglial engulfment capacity encodes maladaptation of ZI V GABA , thus promoting the development of anxiety-like behaviors in acute pain.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Farzinpour, Liu, Cao, Mao, Zhang and Jin.)
Databáze: MEDLINE
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