Simazine Enhances Dark Fermentative H 2 Production by Unicellular Halotolerant Cyanobacterium Aphanothece halophytica .

Autor: Pansook S; Department of Biology, School of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok, Thailand., Incharoensakdi A; Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand., Phunpruch S; Department of Biology, School of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok, Thailand.; Bioenergy Research Unit, School of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok, Thailand.
Jazyk: angličtina
Zdroj: Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2022 Jul 15; Vol. 10, pp. 904101. Date of Electronic Publication: 2022 Jul 15 (Print Publication: 2022).
DOI: 10.3389/fbioe.2022.904101
Abstrakt: The halotolerant cyanobacterium Aphanothece halophytica is a potential H 2 producer that induces H 2 evolution under nitrogen deprivation. H 2 is mainly produced via the catabolism of stored glycogen under dark anaerobic condition. H 2 evolution is catalyzed by O 2 -sensitive bidirectional hydrogenase. The aim of this study was to improve H 2 production by A. halophytica using various kinds of inhibitors. Among all types of inhibitors, simazine efficiently promoted the highest H 2 production under dark conditions. High simazine concentration and long-term incubation resulted in a decrease in cell and chlorophyll concentrations. The optimal simazine concentration for H 2 production by A. halophytica was 25 µM. Simazine inhibited photosynthetic O 2 evolution but promoted dark respiration, resulting in a decrease in O 2 level. Hence, the bidirectional hydrogenase activity and H 2 production was increased. A. halophytica showed the highest H 2 production rate at 58.88 ± 0.22 µmol H 2 g -1 dry weight h -1 and H 2 accumulation at 356.21 ± 6.04 μmol H 2 g -1 dry weight after treatment with 25 µM simazine under dark anaerobic condition for 2 and 24 h, respectively. This study demonstrates the potential of simazine for the enhancement of dark fermentative H 2 production by A. halophytica .
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Pansook, Incharoensakdi and Phunpruch.)
Databáze: MEDLINE
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