Genetic Diversity of Mycobacterium tuberculosis Isolates From an Amerindian Population in Chiapas, México.

Autor:	Molina-Torres CA; Laboratorio Interdisciplinario de Investigación Dermatológica, Servicio de Dermatología, Hospital Universitario, Universidad Autónoma de Nuevo León, Monterrey, Mexico., Quinn FD; Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, United States., Castro-Garza J; Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, Mexico., Gómez-Velasco A; Departamento de Ecología Humana, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Unidad Mérida, Mérida, Mexico., Ocampo-Candiani J; Laboratorio Interdisciplinario de Investigación Dermatológica, Servicio de Dermatología, Hospital Universitario, Universidad Autónoma de Nuevo León, Monterrey, Mexico., Bencomo-Alerm A; Laboratorio de Micobacterias, Programa de Prevención y Control de la Tuberculosis, región Altos de Chiapas, Instituto de Salud del Estado de Chiapas, Secretaría de Salud (SSA), San Cristóbal de Las Casas, Mexico., Sánchez-Pérez HJ; Departamento de Salud, El Colegio de la Frontera Sur (ECOSUR), San Cristóbal de Las Casas, Mexico., Muñoz-Jiménez S; Laboratorio de Micobacterias, Programa de Prevención y Control de la Tuberculosis, región Altos de Chiapas, Instituto de Salud del Estado de Chiapas, Secretaría de Salud (SSA), San Cristóbal de Las Casas, Mexico., Rendón A; Centro de Investigación, Prevención y Tratamiento de Infecciones Respiratorias, Hospital Universitario, Universidad Autónoma de Nuevo León, Monterrey, Mexico., Ansari A; Microbial Pathogenesis and Genomics Lab, ICMR-National Institute of Research in Tribal Health, Jabalpur, India., Sharma M; Microbial Pathogenesis and Genomics Lab, ICMR-National Institute of Research in Tribal Health, Jabalpur, India., Singh P; Microbial Pathogenesis and Genomics Lab, ICMR-National Institute of Research in Tribal Health, Jabalpur, India., Vera-Cabrera L; Laboratorio Interdisciplinario de Investigación Dermatológica, Servicio de Dermatología, Hospital Universitario, Universidad Autónoma de Nuevo León, Monterrey, Mexico.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2022 Jul 13; Vol. 12, pp. 875909. Date of Electronic Publication: 2022 Jul 13 (Print Publication: 2022).
DOI:	10.3389/fcimb.2022.875909
Abstrakt:	This is the first report of the genetic diversity of the Mycobacterium tuberculosis complex isolates found in a Mexican-Amerindian setting. In this study, we analyzed isolates collected from the Highlands region of Chiapas, Mexico, by using spoligotyping and whole-genome sequencing analyses. Seventy-three M. tuberculosis isolates were analyzed initially by spoligotyping; no new spoligotypes were identified. Nineteen percent of the isolates were identified as SIT53 (T1) (n = 14), followed by SIT42 (14%, n = 10, LAM9) and SIT119 (11%; n = 8, X1). SIT53, SIT42, and orphan isolates (16.4%, n = 12) constituted about 50% of the isolates studied and were subjected to whole-genome sequencing (WGS) analysis. Most SIT53 (10/12) isolates belonged to the Euro-American sub-lineage 4.8. Most SIT42 isolates (4/7) as .well as most orphan isolates (5/8) belonged to the lineage 4.3.3 LAM group. By comparing the single-nucleotide polymorphism (SNP) patterns of the SIT53 isolates, we found one clone (<7 SNPs) and four clustered isolates (<15 SNPs). In isolates from the SIT42 and orphan groups, we did not find any clones or clusters. This work demonstrates the success of sub-lineage 4.8 to predominate in Mexico and confirms the dominion of sub-lineage 4.3.3 in Central and South America. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The handling editor KKM declared a shared affiliation with the author(s) AA, MS, and PS at the time of review. (Copyright © 2022 Molina-Torres, Quinn, Castro-Garza, Gómez-Velasco, Ocampo-Candiani, Bencomo-Alerm, Sánchez-Pérez, Muñoz-Jiménez, Rendón, Ansari, Sharma, Singh and Vera-Cabrera.)
Databáze:	MEDLINE
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