Anatomical characterization of vagal nodose afferent innervation and ending morphologies at the murine heart using a transgenic approach.

Autor: Kazci YE; Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, 34810 Istanbul, Turkey; Istanbul Medipol University, Institute of Health Sciences, Neuroscience Program, Istanbul, Turkey; Deparment of Medical Biology, International School of Medicine, Istanbul Medipol University, Istanbul, Turkey., Sahoglu Goktas S; Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, 34810 Istanbul, Turkey; Istanbul Medipol University, Institute of Health Sciences, Neuroscience Program, Istanbul, Turkey; Department of Medical Biology, School of Medicine, Istanbul Medipol University, Istanbul, Turkey., Aydin MS; Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, 34810 Istanbul, Turkey., Karadogan B; Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, 34810 Istanbul, Turkey., Nebol A; Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, 34810 Istanbul, Turkey; Istanbul Medipol University, Institute of Health Sciences, Medical Biology and Genetics Graduate Program, Istanbul, Turkey., Turhan MU; Cerrahpasa Medical School, Department of Cardiovascular Surgery, Istanbul University, Istanbul, Turkey., Ozturk G; Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, 34810 Istanbul, Turkey; Istanbul Medipol University, Institute of Health Sciences, Neuroscience Program, Istanbul, Turkey; Physiology Department, International School of Medicine, Istanbul Medipol University, 34810 Istanbul, Turkey., Cagavi E; Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, 34810 Istanbul, Turkey; Deparment of Medical Biology, International School of Medicine, Istanbul Medipol University, Istanbul, Turkey; Department of Medical Biology, School of Medicine, Istanbul Medipol University, Istanbul, Turkey; Istanbul Medipol University, Institute of Health Sciences, Medical Biology and Genetics Graduate Program, Istanbul, Turkey. Electronic address: ecagavi@medipol.edu.tr.
Jazyk: angličtina
Zdroj: Autonomic neuroscience : basic & clinical [Auton Neurosci] 2022 Nov; Vol. 242, pp. 103019. Date of Electronic Publication: 2022 Jul 12.
DOI: 10.1016/j.autneu.2022.103019
Abstrakt: Heart is an extensively innervated organ and its function is strictly coordinated by autonomic neural circuits. After pathological events such as myocardial infarction (MI), cardiac nerves undergo a structural and functional remodeling contributing to cardiac dysfunction. Although the efferent component of the cardiac nerves has been well described, sensory innervation of the heart has not been defined in detail. Considering its importance, comprehensive description of vagal afferent innervation on the whole heart would enable a better description of autonomic imbalances manifesting as sympathoexcitation and vagal withdrawal in post-ischemic states. To address this issue, we globally mapped the vagal nodose afferent fibers innervating the whole murine heart with unprecedented resolution. By using the Phox2b-Cre::tdTomato transgenic mouse line, we described the detailed distribution and distinct vagal sensory ending morphologies at both the dorsal and ventral sides of the mouse heart. By neural tracing analysis, we quantitated the distribution and prevalence of vagal afferent nerve fibers with varying diameters across dorsal and ventral surfaces of the heart. Moreover, we demonstrated that vagal afferents formed flower spray and end-net-like endings within the atria and ventricles. As distinct from the atria, vagal afferents formed intramuscular array-like endings within the ventricles. Furthermore, we showed that vagal afferents undergo structural remodeling by forming axonal sprouts around the infarct area in post-MI hearts. These findings improve our understanding of the potential effect of vagal afferent remodeling on autonomic imbalance and generation of cardiac arrhythmias and could prospectively contribute to the development of more effective neuromodulatory therapies.
Competing Interests: Declaration of competing interest The authors declare no competing interests.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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