NPC transplantation rescues sci-driven cAMP/EPAC2 alterations, leading to neuroprotection and microglial modulation.

Autor: Martínez-Rojas B; Neuronal and Tissue Regeneration Laboratory, Centro de Investigación Príncipe Felipe, 46012, Valencia, Spain., Giraldo E; Neuronal and Tissue Regeneration Laboratory, Centro de Investigación Príncipe Felipe, 46012, Valencia, Spain.; Department of Biotechnology, Universitat Politècnica de València, Valencia, Spain.; UPV-CIPF Joint Research Unit Disease Mechanisms and Nanomedicine, Centro de Investigación Príncipe Felipe, 46012, Valencia, Spain., Grillo-Risco R; Bioinformatics Unit, Centro de Investigación Príncipe Felipe, 46012, Valencia, Spain., Hidalgo MR; Bioinformatics Unit, Centro de Investigación Príncipe Felipe, 46012, Valencia, Spain., López-Mocholi E; Neuronal and Tissue Regeneration Laboratory, Centro de Investigación Príncipe Felipe, 46012, Valencia, Spain., Alastrue-Agudo A; Neuronal and Tissue Regeneration Laboratory, Centro de Investigación Príncipe Felipe, 46012, Valencia, Spain., García-García F; Bioinformatics Unit, Centro de Investigación Príncipe Felipe, 46012, Valencia, Spain., Moreno-Manzano V; Neuronal and Tissue Regeneration Laboratory, Centro de Investigación Príncipe Felipe, 46012, Valencia, Spain. vmorenom@cipf.es.
Jazyk: angličtina
Zdroj: Cellular and molecular life sciences : CMLS [Cell Mol Life Sci] 2022 Jul 29; Vol. 79 (8), pp. 455. Date of Electronic Publication: 2022 Jul 29.
DOI: 10.1007/s00018-022-04494-w
Abstrakt: Neural progenitor cell (NPC) transplantation represents a promising treatment strategy for spinal cord injury (SCI); however, the underlying therapeutic mechanisms remain incompletely understood. We demonstrate that severe spinal contusion in adult rats causes transcriptional dysregulation, which persists from early subacute to chronic stages of SCI and affects nearly 20,000 genes in total tissue extracts. Functional analysis of this dysregulated transcriptome reveals the significant downregulation of cAMP signalling components immediately after SCI, involving genes such as EPAC2 (exchange protein directly activated by cAMP), PKA, BDNF, and CAMKK2. The ectopic transplantation of spinal cord-derived NPCs at acute or subacute stages of SCI induces a significant transcriptional impact in spinal tissue, as evidenced by the normalized expression of a large proportion of SCI-affected genes. The transcriptional modulation pattern driven by NPC transplantation includes the rescued expression of cAMP signalling genes, including EPAC2. We also explore how the sustained in vivo inhibition of EPAC2 downstream signalling via the intrathecal administration of ESI-05 for 1 week impacts therapeutic mechanisms involved in the NPC-mediated treatment of SCI. NPC transplantation in SCI rats in the presence and absence of ESI-05 administration prompts increased rostral cAMP levels; however, NPC and ESI-05 treated animals exhibit a significant reduction in EPAC2 mRNA levels compared to animals receiving only NPCs treatment. Compared with transplanted animals, NPCs + ESI-05 treatment increases the scar area (as shown by GFAP staining), polarizes microglia into an inflammatory phenotype, and increases the magnitude of the gap between NeuN + cells across the lesion. Overall, our results indicate that the NPC-associated therapeutic mechanisms in the context of SCI involve the cAMP pathway, which reduces inflammation and provides a more neuropermissive environment through an EPAC2-dependent mechanism.
(© 2022. The Author(s).)
Databáze: MEDLINE
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