MLPA as a genetic assay for the prenatal diagnosis of common aneuploidy: the first Egyptian experience.

Autor: Eid OM; Departments of Human Cytogenetics, National Research Centre, El Bohouth Street, Dokki, Cairo, 12311, Egypt. olameid@hotmail.com., Eid MM; Departments of Human Cytogenetics, National Research Centre, El Bohouth Street, Dokki, Cairo, 12311, Egypt., Farid M; Departments of Human Cytogenetics, National Research Centre, El Bohouth Street, Dokki, Cairo, 12311, Egypt., Abdel Kader RMA; Departments of Human Cytogenetics, National Research Centre, El Bohouth Street, Dokki, Cairo, 12311, Egypt., Mahrous R; Departments of Human Cytogenetics, National Research Centre, El Bohouth Street, Dokki, Cairo, 12311, Egypt., El-Dessouky SH; Prenatal Diagnosis & Fetal Medicine, National Research Centre, El Bohouth Street, Dokki, Cairo, 12311, Egypt.
Jazyk: angličtina
Zdroj: Journal, genetic engineering & biotechnology [J Genet Eng Biotechnol] 2022 Jul 28; Vol. 20 (1), pp. 112. Date of Electronic Publication: 2022 Jul 28.
DOI: 10.1186/s43141-022-00402-8
Abstrakt: Background: The prenatal diagnosis of syndromes caused by chromosomal abnormality is a long-established part of obstetric care. Several DNA-based molecular approaches have provided rapid prenatal diagnosis of of cytogenomic abnormalities. MLPA has become available for rapid aneuploidy detection of the most common chromosome abnormalities.
Objectives: The aim of this study is to introduce the MLPA technique as a method for the prenatal detection of aneuploidy in Egypt by its validation compared to the FISH technique.
Methods: Fifty AF samples were collected for this study and were subjected to MLPA and FISH assays to detect the most common prenatal chromosomal abnormality.
Results and Conclusions: Our study confirmed previous reports that MLPA is analogous to FISH for detecting common aneuploidies and could be a quick and dependable tool for prenatal diagnosis. Therefore, initial prompt testing of AF samples for the copy number of the most common occurring aneuploidies is recommended.
(© 2022. The Author(s).)
Databáze: MEDLINE
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