Mitochondrial Ca 2+ Uptake Drives Endothelial Injury By Radiation Therapy.

Autor: Ait-Aissa K; Abboud Cardiovascular Research Center, Department of Internal Medicine (K.A.A., O.M.K., N.R.L., I.M.G.), Carver College of Medicine, University of Iowa., Koval OM; Abboud Cardiovascular Research Center, Department of Internal Medicine (K.A.A., O.M.K., N.R.L., I.M.G.), Carver College of Medicine, University of Iowa., Lindsey NR; Abboud Cardiovascular Research Center, Department of Internal Medicine (K.A.A., O.M.K., N.R.L., I.M.G.), Carver College of Medicine, University of Iowa., Grumbach IM; Abboud Cardiovascular Research Center, Department of Internal Medicine (K.A.A., O.M.K., N.R.L., I.M.G.), Carver College of Medicine, University of Iowa.; Free Radical and Radiation Biology Program, Department of Radiation Oncology (I.M.G.), Carver College of Medicine, University of Iowa.; Iowa City VA Healthcare System, Iowa City (I.M.G.).
Jazyk: angličtina
Zdroj: Arteriosclerosis, thrombosis, and vascular biology [Arterioscler Thromb Vasc Biol] 2022 Sep; Vol. 42 (9), pp. 1121-1136. Date of Electronic Publication: 2022 Jul 28.
DOI: 10.1161/ATVBAHA.122.317869
Abstrakt: Background: Radiation therapy strongly increases the risk of atherosclerotic vascular disease, such as carotid stenosis. Radiation induces DNA damage, in particular in mitochondria, but the upstream and downstream signaling events are poorly understood. The objective of this study was to define such mechanisms.
Methods: Endothelial-specific MCU (mitochondrial Ca 2+ uniporter) knockout and C57Bl6/J mice with or without a preinfusion of a mitoTEMPO (mitochondrial reactive oxygen species [ROS] scavenger) were exposed to a single dose of cranial irradiation. 24, and 240 hours postirradiation, vascular reactivity, endothelial function, and mitochondrial integrity were assessed ex vivo and in vitro.
Results: In cultured human endothelial cells, irradiation with 4 Gy increased cytosolic Ca 2+ transients and the mitochondrial Ca 2+ concentration ([Ca 2+ ] mt ) and activated MCU. These outcomes correlated with increases in mitochondrial ROS ( mt ROS), loss of NO production, and sustained damage to mitochondrial but not nuclear DNA. Moreover, irradiation impaired activity of the ETC (electron transport chain) and the transcription of ETC subunits encoded by mitochondrial DNA ( mt DNA). Knockdown or pharmacological inhibition of MCU blocked irradiation-induced mt ROS production, mt DNA damage, loss of NO production, and impairment of ETC activity. Similarly, the pretreatment with mitoTEMPO, a scavenger of mt ROS, reduced irradiation-induced Ca 2+ entry, and preserved both the integrity of the mt DNA and the production of NO, suggesting a feed-forward loop involving [Ca 2+ ] m and mt ROS. Enhancement of DNA repair in mitochondria, but not in the nucleus, was sufficient to block prolonged mt ROS elevations and maintain NO production. Consistent with the findings from cultured cells, in C57BL/6J mice, head and neck irradiation decreased endothelium-dependent vasodilation, and mt DNA integrity in the carotid artery after irradiation. These effects were prevented by endothelial knockout of MCU or infusion with mitoTEMPO.
Conclusions: Irradiation-induced damage to mt DNA is driven by MCU-dependent Ca 2+ influx and the generation of mt ROS. Such damage leads to reduced transcription of mitochondrial genes and activity of the ETC, promoting sustained mt ROS production that induces endothelial dysfunction. Our findings suggest that targeting MCU and mt ROS might be sufficient to mitigate irradiation-induced vascular disease.
Databáze: MEDLINE
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