Untargeted metabolomic analysis of thoracic blood from badgers indicate changes linked to infection with bovine tuberculosis (Mycobacterium bovis): a pilot study.

Autor: Bauman JS; Institute of Biological, Environmental and Rural Science, Aberystwyth University, Aberystwyth, SY23 3DA, Ceredigion, UK., Pizzey R; Institute of Biological, Environmental and Rural Science, Aberystwyth University, Aberystwyth, SY23 3DA, Ceredigion, UK., Beckmann M; Institute of Biological, Environmental and Rural Science, Aberystwyth University, Aberystwyth, SY23 3DA, Ceredigion, UK., Villarreal-Ramos B; Institute of Biological, Environmental and Rural Science, Aberystwyth University, Aberystwyth, SY23 3DA, Ceredigion, UK.; Centre of Excellence for Bovine Tuberculosis, Aberystwyth University, Aberystwyth, SY23 3AR, Ceredigion, UK.; TB Research Group, Animal and Plant Health Agency, New Haw, Addlestone, KT15 3NB, Surrey, UK., King J; Wales Veterinary Science Centre, Y Buarth, Aberystwyth, SY23 1ND, Ceredigion, UK., Hopkins B; Wales Veterinary Science Centre, Y Buarth, Aberystwyth, SY23 1ND, Ceredigion, UK., Rooke D; ProTEM Services Ltd, West Sussex, UK., Hewinson G; Institute of Biological, Environmental and Rural Science, Aberystwyth University, Aberystwyth, SY23 3DA, Ceredigion, UK.; Centre of Excellence for Bovine Tuberculosis, Aberystwyth University, Aberystwyth, SY23 3AR, Ceredigion, UK., Mur LAJ; Institute of Biological, Environmental and Rural Science, Aberystwyth University, Aberystwyth, SY23 3DA, Ceredigion, UK. lum@aber.ac.uk.; Aberystwyth University, B2.03 Edward Llwyd, Penglais, Aberystwyth, SY23 3FL, UK. lum@aber.ac.uk.
Jazyk: angličtina
Zdroj: Metabolomics : Official journal of the Metabolomic Society [Metabolomics] 2022 Jul 27; Vol. 18 (8), pp. 61. Date of Electronic Publication: 2022 Jul 27.
DOI: 10.1007/s11306-022-01915-6
Abstrakt: Introduction: Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB) in cattle, represents a major disease burden to UK cattle farming, with considerable costs associated with its control. The European badger (Meles meles) is a known wildlife reservoir for bTB and better knowledge of the epidemiology of bTB through testing wildlife is required for disease control. Current tests available for the diagnosis of bTB in badgers are limited by cost, processing time or sensitivities.
Materials and Methods: We assessed the ability of flow infusion electrospray-high-resolution mass spectrometry (FIE-HRMS) to determine potential differences between infected and non-infected badgers based on thoracic blood samples obtained from badgers found dead in Wales. Thoracic blood samples were autoclaved for handling in a containment level 2 (CL2) hazard laboratory.
Results: Here we show the major differences associated with with M. bovis infection were changes to folate, pyrimidine, histidine, glycerophospholipid and phosphonate metabolism.
Conclusions: Our studies have indicated differences in the metabolomic signature of badgers found dead in relation to their infection status, suggesting metabolomics could hold potential for developing novel diagnostics for bTB in badgers. As well as highlighting a potential way to handle samples containing a highly pathogenic agent at CL2 for metabolomics studies.
(© 2022. The Author(s).)
Databáze: MEDLINE
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