Potential diagnostic biomarkers for pulmonary tuberculosis in humans are not elevated in Mycobacterium tuberculosis culture-positive Asian elephants (Elephas maximus).

Autor:	Coughlin LL; Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA., Sanchez CR; Veterinary Medical Center, Oregon Zoo, Portland, OR., Monti MI; Veterinary Medical Center, Oregon Zoo, Portland, OR., Griffioen JA; Fort Worth Zoo, Fort Worth, TX., Nutter FB; Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA., Beamer GL; Department of Infectious Disease and Global Health, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA.
Jazyk:	angličtina
Zdroj:	American journal of veterinary research [Am J Vet Res] 2022 Jun 21; Vol. 83 (8). Date of Electronic Publication: 2022 Jun 21.
DOI:	10.2460/ajvr.22.01.0016
Abstrakt:	Objective: To determine (1) if chemokine (C-X-C motif) ligand 1 (CXCL1), matrix metalloproteinase 8 (MMP8), interleukin-10 (IL-10), interferon-γ (IFN-γ), and tumor necrosis factor-α (TNF-α) can be detected in serum from Asian elephants, and (2) if their concentrations are significantly elevated in Mycobacterium tuberculosis (M.tb) culture-positive elephants compared to -negative elephants. CXCL1, MMP8, IL-10, IFN-γ, and TNF-α were recently identified as potential diagnostic biomarkers for pulmonary tuberculosis in experimental studies in animals and humans. Therefore, we hypothesized that they would be detectable and significantly elevated in M.tb culture-positive elephants compared to M.tb culture-negative elephants. Sample: 101 Asian elephant serum samples, including 91 samples from 6 M.tb-negative elephants and 10 samples from 5 M.tb-positive elephants (none of which exhibited clinical signs of disease). M.tb status was determined by trunk wash culture. Procedures: Commercially available ELISA kits were used to determine the concentrations of each biomarker in serum samples. Results: Biomarker concentrations were below the limit of detection for the assay in 100/101 (99%) samples for CXCL1, 98/101 (97%) samples for MMP8, 85/101 (84%) samples for IL-10, 75/101 (74%) samples for IFN-γ, and 45/101 (45%) samples for TNF-α. Multiple M.tb culture-positive elephants did not have detectable levels of any of the 5 biomarkers. Clinical Relevance: CXCL1, MMP8, IL-10, IFN-γ, and TNF-α were not elevated in M.tb culture-positive Asian elephants compared to M.tb culture-negative Asian elephants. This may be related to disease state (ie, clinically asymptomatic). More sensitive assays are needed to better understand the role of these biomarkers in M.tb infection in Asian elephants.
Databáze:	MEDLINE
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