Sulfated Glucan from the Green Seaweed Caulerpa sertularioides Inhibits Adipogenesis through Suppression of Adipogenic and Lipogenic Key Factors.

Autor: Chaves Filho GP; Laboratory of Molecular and Genomic Biology, Department of Biology and Genetics, Center of Biosciences, Federal University of Rio Grande do Norte, Natal 59072-900, RN, Brazil.; The Doctoral Program in Biotechnology-Northeast Biotechnology Network (RENORBIO), Center of Biosciences, Federal University of Rio Grande do Norte, Natal 59072-900, RN, Brazil., Batista LANC; Laboratory of Biotechnology of Natural Polymers, Department of Biochemistry, Center of Biosciences, Federal University of Rio Grande do Norte, Natal 59072-900, RN, Brazil., de Medeiros SRB; Laboratory of Molecular and Genomic Biology, Department of Biology and Genetics, Center of Biosciences, Federal University of Rio Grande do Norte, Natal 59072-900, RN, Brazil.; The Doctoral Program in Biotechnology-Northeast Biotechnology Network (RENORBIO), Center of Biosciences, Federal University of Rio Grande do Norte, Natal 59072-900, RN, Brazil., Rocha HAO; The Doctoral Program in Biotechnology-Northeast Biotechnology Network (RENORBIO), Center of Biosciences, Federal University of Rio Grande do Norte, Natal 59072-900, RN, Brazil.; Laboratory of Biotechnology of Natural Polymers, Department of Biochemistry, Center of Biosciences, Federal University of Rio Grande do Norte, Natal 59072-900, RN, Brazil., Moreira SMG; Laboratory of Molecular and Genomic Biology, Department of Biology and Genetics, Center of Biosciences, Federal University of Rio Grande do Norte, Natal 59072-900, RN, Brazil.; The Doctoral Program in Biotechnology-Northeast Biotechnology Network (RENORBIO), Center of Biosciences, Federal University of Rio Grande do Norte, Natal 59072-900, RN, Brazil.
Jazyk: angličtina
Zdroj: Marine drugs [Mar Drugs] 2022 Jul 23; Vol. 20 (8). Date of Electronic Publication: 2022 Jul 23.
DOI: 10.3390/md20080470
Abstrakt: Sulfated polysaccharides (SPS) from seaweeds have great biochemical and biotechnological potential. This study aimed to investigate the effect of SPS isolated from the seaweed Caulerpa sertularioides on adipogenic differentiation as a possible alternative treatment for obesity. The SPS-rich extract from the seaweed C. sertularioides was fractioned into three SPS-rich fractions (F0.5; F0.9; and F1.8) chemically characterized. Among these four samples, only F0.9 showed a significant inhibitory effect on adipogenesis of 3T3-L1 preadipocytes. Ten SPS-rich fractions were isolated from F0.9 through ion-exchange chromatography. However, only the fraction (CS0.2) containing a sulfated glucan was able to inhibit adipogenesis. CS0.2 reduces lipid accumulation and inhibits the expression of key adipogenic (PPARγ, C/EBPβ, and C/EBPα) and lipogenic markers (SREBP-1c, Fabp4, and CD36). The data points to the potential of sulfated glucan from C. sertularioides for the development of functional approaches in obesity management.
Databáze: MEDLINE