| Autor: |
Truong Nguyen PT; Department of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, Finland., Culverwell CL; Department of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, Finland.; The Natural History Museum, Cromwell Road, South Kensington, London SW5 7BD, UK., Suvanto MT; Department of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, Finland.; Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöbergin Katu 2, P.O. Box 66, FI-00014 Helsinki, Finland., Korhonen EM; Department of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, Finland.; Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöbergin Katu 2, P.O. Box 66, FI-00014 Helsinki, Finland., Uusitalo R; Department of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, Finland.; Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöbergin Katu 2, P.O. Box 66, FI-00014 Helsinki, Finland.; Department of Geosciences and Geography, Faculty of Science, University of Helsinki, Gustaf Hällströmin Katu 2, P.O. Box 64, FI-00014 Helsinki, Finland., Vapalahti O; Department of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, Finland.; Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöbergin Katu 2, P.O. Box 66, FI-00014 Helsinki, Finland.; Virology and Immunology, Diagnostic Center, HUSLAB, Helsinki University Hospital, FI-00029 Helsinki, Finland., Smura T; Department of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, Finland., Huhtamo E; Department of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, Finland.; Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöbergin Katu 2, P.O. Box 66, FI-00014 Helsinki, Finland. |
| Abstrakt: |
RNA viromes of nine commonly encountered Ochlerotatus mosquito species collected around Finland in 2015 and 2017 were studied using next-generation sequencing. Mosquito homogenates were sequenced from 91 pools comprising 16-60 morphologically identified adult females of Oc. cantans , Oc. caspius , Oc. communis , Oc. diantaeus , Oc. excrucians , Oc. hexodontus , Oc. intrudens , Oc. pullatus and Oc. punctor / punctodes . In total 514 viral Reverse dependent RNA polymerase (RdRp) sequences of 159 virus species were recovered, belonging to 25 families or equivalent rank, as follows: Aliusviridae , Aspiviridae , Botybirnavirus , Chrysoviridae , Chuviridae , Endornaviridae , Flaviviridae , Iflaviridae , Negevirus , Partitiviridae , Permutotetraviridae , Phasmaviridae , Phenuiviridae , Picornaviridae , Qinviridae , Quenyavirus , Rhabdoviridae , Sedoreoviridae , Solemoviridae , Spinareoviridae , Togaviridae , Totiviridae , Virgaviridae , Xinmoviridae and Yueviridae . Of these, 147 are tentatively novel viruses. One sequence of Sindbis virus , which causes Pogosta disease in humans, was detected from Oc. communis from Pohjois-Karjala. This study greatly increases the number of mosquito-associated viruses known from Finland and presents the northern-most mosquito-associated viruses in Europe to date. |
