| Autor: |
Fatoba AJ; Discipline of Genetics, School of Life Sciences, University of KwaZulu-Natal, Westville Campus, P/Bag X54001, Durban 4000, South Africa., Adeleke VT; Department of Chemical Engineering, Mangosuthu University of Technology, Umlazi, Durban 4031, South Africa., Maharaj L; Discipline of Genetics, School of Life Sciences, University of KwaZulu-Natal, Westville Campus, P/Bag X54001, Durban 4000, South Africa., Okpeku M; Discipline of Genetics, School of Life Sciences, University of KwaZulu-Natal, Westville Campus, P/Bag X54001, Durban 4000, South Africa., Adeniyi AA; Department of Industrial Chemistry, Federal University, Oye-Ekiti P.O. Box 370111, Nigeria.; Department of Chemistry, University of the Free State, P.O. Box 339, Bloemfontein 9300, South Africa., Adeleke MA; Discipline of Genetics, School of Life Sciences, University of KwaZulu-Natal, Westville Campus, P/Bag X54001, Durban 4000, South Africa. |
| Abstrakt: |
Chicken anemia virus (CAV) causes severe clinical and sub-clinical infection in poultry globally and thus leads to economic losses. The drawbacks of the commercially available vaccines against CAV disease signal the need for a novel, safe, and effective vaccine design. In this study, a multiepitope vaccine (MEV) consisting of T-cell and B-cell epitopes from CAV viral proteins (VP1 and VP2) was computationally constructed with the help of linkers and adjuvant. The 3D model of the MEV construct was refined and validated by different online bioinformatics tools. Molecular docking showed stable interaction of the MEV construct with TLR3, and this was confirmed by Molecular Dynamics Simulation. Codon optimization and in silico cloning of the vaccine in pET-28a (+) vector also showed its potential expression in the E. coli K12 system. The immune simulation also indicated the ability of this vaccine to induce an effective immune response against this virus. Although the vaccine in this study was computationally constructed and still requires further in vivo study to confirm its effectiveness, this study marks a very important step towards designing a potential vaccine against CAV disease. |
