Development and Validation of Indirect Enzyme-Linked Immunosorbent Assays for Detecting Antibodies to SARS-CoV-2 in Cattle, Swine, and Chicken.

Autor: Gontu A; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA., Marlin EA; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.; Clinical & Diagnostic Assay Development Group, Pfizer, Pearl River, NY 10965, USA., Ramasamy S; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA., Neerukonda S; U.S. Department of Health and Human Services, Silver Spring, MD 20993, USA., Anil G; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA., Morgan J; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA., Quraishi M; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA., Chen C; Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USA., Boorla VS; Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USA., Nissly RH; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA., Jakka P; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA., Chothe SK; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA., Ravichandran A; Department of Integrative and Biomedical Physiology, The Pennsylvania State University, University Park, PA 16802, USA., Kodali N; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.; Huck Institute of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA., Amirthalingam S; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.; Huck Institute of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA., LaBella L; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA., Kelly K; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA., Natesan P; Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600007, India., Minns AM; Huck Institute of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.; Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA., Rossi RM; Huck Institute of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA., Werner JR; Department of Animal Science, The Pennsylvania State University, University Park, PA 16802, USA., Hovingh E; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA., Lindner SE; Huck Institute of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.; Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA., Tewari D; Pennsylvania Department of Agriculture, Pennsylvania Veterinary Laboratory, Harrisburg, PA 17110, USA., Kapur V; Huck Institute of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.; Department of Animal Science, The Pennsylvania State University, University Park, PA 16802, USA.; Center for Infectious Disease Dynamics, The Pennsylvania State University, University Park, PA 16802, USA., Vandegrift KJ; Huck Institute of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.; Center for Infectious Disease Dynamics, The Pennsylvania State University, University Park, PA 16802, USA.; Department of Biology, The Pennsylvania State University, University Park, PA 16802, USA., Maranas CD; Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USA., Surendran Nair M; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA., Kuchipudi SV; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA.; Huck Institute of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.; Center for Infectious Disease Dynamics, The Pennsylvania State University, University Park, PA 16802, USA.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2022 Jun 22; Vol. 14 (7). Date of Electronic Publication: 2022 Jun 22.
DOI: 10.3390/v14071358
Abstrakt: Multiple domestic and wild animal species are susceptible to SARS-CoV-2 infection. Cattle and swine are susceptible to experimental SARS-CoV-2 infection. The unchecked transmission of SARS-CoV-2 in animal hosts could lead to virus adaptation and the emergence of novel variants. In addition, the spillover and subsequent adaptation of SARS-CoV-2 in livestock could significantly impact food security as well as animal and public health. Therefore, it is essential to monitor livestock species for SARS-CoV-2 spillover. We developed and optimized species-specific indirect ELISAs (iELISAs) to detect anti-SARS-CoV-2 antibodies in cattle, swine, and chickens using the spike protein receptor-binding domain (RBD) antigen. Serum samples collected prior to the COVID-19 pandemic were used to determine the cut-off threshold. RBD hyperimmunized sera from cattle ( n = 3), swine ( n = 6), and chicken ( n = 3) were used as the positive controls. The iELISAs were evaluated compared to a live virus neutralization test using cattle ( n = 150), swine ( n = 150), and chicken ( n = 150) serum samples collected during the COVID-19 pandemic. The iELISAs for cattle, swine, and chicken were found to have 100% sensitivity and specificity. These tools facilitate the surveillance that is necessary to quickly identify spillovers into the three most important agricultural species worldwide.
Databáze: MEDLINE
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