| Autor: |
Mannucci A; Department of Agriculture, Food and Environment, University of Pisa, 56124 Pisa, Italy.; Laboratory of Functional Plant Biology, Department of Biology, Faculty of Sciences, Ghent University, 9000 Ghent, Belgium., Santin M; Department of Agriculture, Food and Environment, University of Pisa, 56124 Pisa, Italy., Vanhaelewyn L; Laboratory of Functional Plant Biology, Department of Biology, Faculty of Sciences, Ghent University, 9000 Ghent, Belgium.; Department of Agricultural Economics, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.; Deroose Plants NV, Weststraat 129A, 9940 Evergem, Belgium., Sciampagna MC; Department of Agriculture, Food and Environment, University of Pisa, 56124 Pisa, Italy., Miras-Moreno MB; Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, 29122 Piacenza, Italy., Zhang L; Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, 29122 Piacenza, Italy., Lucini L; Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, 29122 Piacenza, Italy., Quartacci MF; Department of Agriculture, Food and Environment, University of Pisa, 56124 Pisa, Italy., Van Der Straeten D; Laboratory of Functional Plant Biology, Department of Biology, Faculty of Sciences, Ghent University, 9000 Ghent, Belgium., Castagna A; Department of Agriculture, Food and Environment, University of Pisa, 56124 Pisa, Italy., Ranieri A; Department of Agriculture, Food and Environment, University of Pisa, 56124 Pisa, Italy. |
| Abstrakt: |
Tomato ( Solanum lycopersicum L.) is globally recognised as a high-value crop both for commercial profit and nutritional benefits. In contrast to the extensive data regarding the changes in the metabolism of tomato fruit exposed to UV radiation, less is known about the foliar and root metabolome. Using an untargeted metabolomic approach through UHPLC-ESI-QTOF-MS analysis, we detected thousands of metabolites in the leaves (3000) and roots (2800) of Micro-Tom tomato plants exposed to 11 days of short daily UV radiation, applied only on the aboveground organs. Multivariate statistical analysis, such as OPLS-DA and volcano, were performed to allow a better understanding of the modifications caused by the treatment. Based on the unexpected modulation to the secondary metabolism, especially the phenylpropanoid pathway, of which compounds were down and up accumulated respectively in leaves and roots of treated plants, a phenolic profiling was carried out for both organs. The phenolic profile was associated with a gene expression analysis to check the transcription trend of genes involved in the UVR8 signalling pathway and the early steps of the phenolic biosynthesis. The retention of the modifications at metabolic and phenolic levels was also investigated 3 days after the UV treatment, showing a prolonged effect on the modulation once the UV treatment had ceased. |
