| Autor: |
Zhang X; State Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin 150040, China.; Department of Biology, Genetics Institute, University of Florida, Gainesville, FL 32610, USA., Tan B; Department of Biology, Genetics Institute, University of Florida, Gainesville, FL 32610, USA., Cheng Z; State Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin 150040, China., Zhu D; Department of Biology, Genetics Institute, University of Florida, Gainesville, FL 32610, USA.; Department of Plant Biology, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China., Jiang T; State Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin 150040, China., Chen S; Department of Biology, Genetics Institute, University of Florida, Gainesville, FL 32610, USA.; Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32610, USA.; Proteomics and Mass Spectrometry, University of Florida, Gainesville, FL 32610, USA. |
| Abstrakt: |
Mesembryanthemum crystallinum (common ice plant) is one of the facultative halophyte plants, and it serves as a model for investigating the molecular mechanisms underlying its salt stress response and tolerance. Here we cloned one of the homeobox transcription factor (TF) genes, McHB7 , from the ice plant, which has 60% similarity with the Arabidopsis AtHB7 . Overexpression of the McHB7 in Arabidopsis (OE) showed that the plants had significantly elevated relative water content (RWC), chlorophyll content, superoxide dismutase (SOD), and peroxidase (POD) activities after salt stress treatment. Our proteomic analysis identified 145 proteins to be significantly changed in abundance, and 66 were exclusively increased in the OE plants compared to the wild type (WT). After salt treatment, 979 and 959 metabolites were significantly increased and decreased, respectively, in the OE plants compared to the WT. The results demonstrate that the McHB7 can improve photosynthesis, increase the leaf chlorophyll content, and affect the TCA cycle by regulating metabolites (e.g., pyruvate) and proteins (e.g., citrate synthase). Moreover, Mc HB7 modulates the expression of stress-related proteins (e.g., superoxide dismutase, dehydroascorbate reductase, and pyrroline-5-carboxylate synthase B) to scavenge reactive oxygen species and enhance plant salt tolerance. |
