| Autor: |
Di Vincenzo M; Department of Clinical and Molecular Sciences-Histology, Università Politecnica delle Marche, 60126 Ancona, Italy., De Quattro C; Department of Biotechnology, University of Verona, 37134 Verona, Italy., Rossato M; Department of Biotechnology, University of Verona, 37134 Verona, Italy., Lazzarini R; Department of Clinical and Molecular Sciences-Histology, Università Politecnica delle Marche, 60126 Ancona, Italy., Delli Carpini G; Clinic of Obstetrics and Gynecology, Department of Clinical Sciences, Università Politecnica delle Marche, 60126 Ancona, Italy., Ciavattini A; Clinic of Obstetrics and Gynecology, Department of Clinical Sciences, Università Politecnica delle Marche, 60126 Ancona, Italy., Orciani M; Department of Clinical and Molecular Sciences-Histology, Università Politecnica delle Marche, 60126 Ancona, Italy. |
| Abstrakt: |
The aetiology of leiomyoma is debated; however, dysregulated progenitor cells or miRNAs appear to be involved. Previous profiling analysis of miRNA in healthy myometrium- (M-MSCs) and leiomyoma- (L-MSCs) derived mesenchymal stem cells (MSCs) identified 15 miRNAs differentially expressed between M-MSCs and L-MSCs. Here, we try to elucidate whether these differentially regulated 15 miRNAs arise as a conversion of M-MSCs along the differentiation process or whether they may originate from divergent cell commitment. To trace the origin of the dysregulation, a comparison was made of the expression of miRNAs previously identified as differentially regulated in M-MSCs and L-MSCs with that detected in MSCs from amniotic fluid (considered as a substitute for embryonic cells). The results do not allow for a foregone conclusion: the miRNAs converging to the adherens junction pathway showed a gradual change along the differentiation process, and the miRNAs which coincided with the other three pathways (ECM-receptor interaction, TGFβ and cell cycle) showed a complex, not linear, regulation and, therefore, a trend along the hypothetical differentiation process was not deduced. However, the role of miRNAs appears to be predominant in the onset of leiomyoma and may follow two different mechanisms (early commitment; exacerbation); furthermore, miRNAs can support the observed (epigenetic) predisposition. |
