Characterization of the Tumor Microenvironment and the Biological Processes with a Role in Prostatic Tumorigenesis.

Autor:	Ionescu CA; Chemical Carcinogenesis and Molecular Biology Laboratory, Institute of Oncology 'Prof. Dr. Alexandru Trestioreanu', 022328 Bucharest, Romania.; Medicine Faculty, 'Ovidius' University of Constanta, 1 Universitatii Street, 900470 Constanta, Romania., Aschie M; Medicine Faculty, 'Ovidius' University of Constanta, 1 Universitatii Street, 900470 Constanta, Romania.; Center for Research and Development of the Morphological and Genetic Studies of Malignant Pathology, 'Ovidius' University of Constanta, 145 Tomis Blvd., 900591 Constanta, Romania.; Clinical Service of Pathology, 'Sf. Apostol Andrei' Emergency County Hospital, 145 Tomis Blvd., 900591 Constanta, Romania., Matei E; Center for Research and Development of the Morphological and Genetic Studies of Malignant Pathology, 'Ovidius' University of Constanta, 145 Tomis Blvd., 900591 Constanta, Romania., Cozaru GC; Center for Research and Development of the Morphological and Genetic Studies of Malignant Pathology, 'Ovidius' University of Constanta, 145 Tomis Blvd., 900591 Constanta, Romania.; Clinical Service of Pathology, 'Sf. Apostol Andrei' Emergency County Hospital, 145 Tomis Blvd., 900591 Constanta, Romania., Deacu M; Medicine Faculty, 'Ovidius' University of Constanta, 1 Universitatii Street, 900470 Constanta, Romania.; Clinical Service of Pathology, 'Sf. Apostol Andrei' Emergency County Hospital, 145 Tomis Blvd., 900591 Constanta, Romania., Mitroi AF; Center for Research and Development of the Morphological and Genetic Studies of Malignant Pathology, 'Ovidius' University of Constanta, 145 Tomis Blvd., 900591 Constanta, Romania.; Clinical Service of Pathology, 'Sf. Apostol Andrei' Emergency County Hospital, 145 Tomis Blvd., 900591 Constanta, Romania., Baltatescu GI; Center for Research and Development of the Morphological and Genetic Studies of Malignant Pathology, 'Ovidius' University of Constanta, 145 Tomis Blvd., 900591 Constanta, Romania.; Clinical Service of Pathology, 'Sf. Apostol Andrei' Emergency County Hospital, 145 Tomis Blvd., 900591 Constanta, Romania., Nicolau AA; Center for Research and Development of the Morphological and Genetic Studies of Malignant Pathology, 'Ovidius' University of Constanta, 145 Tomis Blvd., 900591 Constanta, Romania.; Clinical Service of Pathology, 'Sf. Apostol Andrei' Emergency County Hospital, 145 Tomis Blvd., 900591 Constanta, Romania., Mazilu L; Medicine Faculty, 'Ovidius' University of Constanta, 1 Universitatii Street, 900470 Constanta, Romania.; Oncology Department, 'Sf. Apostol Andrei' Emergency County Hospital, 145 Tomis Blvd., 900591 Constanta, Romania., Tuta LA; Medicine Faculty, 'Ovidius' University of Constanta, 1 Universitatii Street, 900470 Constanta, Romania.; Nephrology Department, 'Sf. Apostol Andrei' Emergency County Hospital, 145 Tomis Blvd., 900591 Constanta, Romania., Iorga IC; Medicine Faculty, 'Ovidius' University of Constanta, 1 Universitatii Street, 900470 Constanta, Romania.; Urology Department, 'Sf. Apostol Andrei' Emergency County Hospital, 145 Tomis Blvd., 900591 Constanta, Romania., Stanigut A; Medicine Faculty, 'Ovidius' University of Constanta, 1 Universitatii Street, 900470 Constanta, Romania.; Nephrology Department, 'Sf. Apostol Andrei' Emergency County Hospital, 145 Tomis Blvd., 900591 Constanta, Romania., Enciu M; Medicine Faculty, 'Ovidius' University of Constanta, 1 Universitatii Street, 900470 Constanta, Romania.; Clinical Service of Pathology, 'Sf. Apostol Andrei' Emergency County Hospital, 145 Tomis Blvd., 900591 Constanta, Romania.
Jazyk:	angličtina
Zdroj:	Biomedicines [Biomedicines] 2022 Jul 12; Vol. 10 (7). Date of Electronic Publication: 2022 Jul 12.
DOI:	10.3390/biomedicines10071672
Abstrakt:	Prostate intratumoral heterogeneity, driven by epithelial−mesenchymal plasticity, contributes to the limited treatment response, and it is therefore necessary to use the biomarkers to improve patient prognostic survival. We aimed to characterize the tumor microenvironment (T lymphocyte infiltration, intratumoral CD34, and KI-67 expressions) by immunohistochemistry methods and to study the biological mechanisms (cell cycle, cell proliferation by adhesion glycoproteins, cell apoptosis) involved in the evolution of the prostate tumor process by flow-cytometry techniques. Our results showed that proliferative activity (S-phase) revealed statistically significant lower values of prostate adenocarcinoma (PCa) and benign prostatic hyperplasia (BPH) reported at non-malignant adjacent cell samples (PCa 4.32 ± 4.91; BPH 2.35 ± 1.37 vs. C 10.23 ± 0.43, p < 0.01). Furthermore, 68% of BPH cases and 88% of patients with PCa had aneuploidy. Statistically increased values of cell proliferation (CD34+ CD61+) were observed in prostate adenocarcinoma and hyperplasia cases reported to non-malignant adjacent cell samples (PCa 28.79 ± 10.14; BPH 40.65 ± 11.88 vs. C 16.15 ± 2.58, p < 0.05). The CD42b+ cell population with a role in cell adhesion, and metastasis had a significantly increased value in PCa cases (38.39 ± 11.23) reported to controls (C 26.24 ± 0.62, p < 0.01). The intratumoral expression of CD34 showed a significantly increased pattern of PCa tissue samples reported to controls (PCa 26.12 ± 6.84 vs. C 1.50 ± 0.70, p < 0.01). Flow cytometric analysis of the cell cycle, apoptosis, and adhesion glycoproteins with a critical role in tumoral cell proliferation, T cell infiltrations, Ki-67, and CD 34 expressions by IHC methods are recommended as techniques for the efficient means of measurement for adenocarcinoma and hyperplasia prostate tissue samples and should be explored in the future.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu